Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   EM854_RS09545 Genome accession   NZ_CP034738
Coordinates   1938974..1939711 (+) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli strain L65     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1933974..1944711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EM854_RS09530 (EM854_09530) clpC 1934428..1937001 (-) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator
  EM854_RS09535 (EM854_09535) yfiH 1937131..1937862 (-) 732 WP_000040115.1 purine nucleoside phosphorylase YfiH -
  EM854_RS09540 (EM854_09540) rluD 1937859..1938839 (-) 981 WP_000079112.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EM854_RS09545 (EM854_09545) comL 1938974..1939711 (+) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  EM854_RS09550 (EM854_09550) raiA 1939982..1940323 (+) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  EM854_RS09555 (EM854_09555) pheL 1940427..1940474 (+) 48 WP_001386991.1 pheA operon leader peptide PheL -
  EM854_RS09560 (EM854_09560) pheA 1940573..1941733 (+) 1161 WP_000200098.1 bifunctional chorismate mutase/prephenate dehydratase -
  EM854_RS09565 (EM854_09565) tyrA 1941776..1942897 (-) 1122 WP_000225221.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  EM854_RS09570 (EM854_09570) aroF 1942908..1943978 (-) 1071 WP_001168054.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  EM854_RS09575 (EM854_09575) yfiL 1944188..1944553 (+) 366 WP_000976004.1 DUF2799 domain-containing protein -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=290595 EM854_RS09545 WP_000197686.1 1938974..1939711(+) (comL) [Escherichia coli strain L65]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=290595 EM854_RS09545 WP_000197686.1 1938974..1939711(+) (comL) [Escherichia coli strain L65]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCGCAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCAAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGAATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376