Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   CSC28_RS26930 Genome accession   NZ_CP029093
Coordinates   5329887..5331479 (+) Length   530 a.a.
NCBI ID   WP_058129588.1    Uniprot ID   A0A2R3IWC5
Organism   Pseudomonas paraeruginosa strain AR441     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5324887..5336479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC28_RS26910 (CSC28_5316) pgeF 5326640..5327368 (-) 729 WP_034081438.1 peptidoglycan editing factor PgeF -
  CSC28_RS26915 (CSC28_5317) rluD 5327365..5328327 (-) 963 WP_003150035.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  CSC28_RS26920 (CSC28_5318) - 5328473..5329498 (+) 1026 WP_012077305.1 outer membrane protein assembly factor BamD -
  CSC28_RS26925 (CSC28_5319) - 5329652..5329897 (+) 246 WP_003150030.1 PP0621 family protein -
  CSC28_RS26930 (CSC28_5320) pilS 5329887..5331479 (+) 1593 WP_058129588.1 two-component system sensor histidine kinase PilS Regulator
  CSC28_RS26935 (CSC28_5321) pilR 5331495..5332832 (+) 1338 WP_033999343.1 two-component system response regulator PilR Regulator
  CSC28_RS26940 (CSC28_5322) thiO 5332878..5333972 (-) 1095 WP_058146146.1 glycine oxidase ThiO -
  CSC28_RS26945 (CSC28_5323) - 5334107..5334616 (+) 510 WP_003150026.1 GspH/FimT family protein -
  CSC28_RS26950 (CSC28_5324) - 5334722..5335228 (+) 507 WP_012077308.1 Tfp pilus assembly protein FimT/FimU -
  CSC28_RS26955 (CSC28_5325) pilV 5335219..5335776 (+) 558 WP_034081446.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59121.34 Da        Isoelectric Point: 7.0317

>NTDB_id=290450 CSC28_RS26930 WP_058129588.1 5329887..5331479(+) (pilS) [Pseudomonas paraeruginosa strain AR441]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEEQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLLLCGLFYAGGGMPSGIGSLLVVAVAIANILLRGRIGLVIAAVASLGLLYLTFFLSLSSPDAPNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSHLSILLANQAALGLLRQDQVRGASLD
RYSPMLVHCMKQWRLNPSRRPPTLKVIPEGPAVQPSFISLNREHDRHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDGADRRLTQIIQDQSRRMNLVIENVLQLSRRRQAEPQQLDLKDWLQRFVDEYPGKLRND
SELHLQLGAGDIHTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDADSDLPVLEIADDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=290450 CSC28_RS26930 WP_058129588.1 5329887..5331479(+) (pilS) [Pseudomonas paraeruginosa strain AR441]
GTGCGCGCTGAGCGGCTGCGGCTGAGCGAGGAGCAAGGGCAGCGCATCCTCCGCCTGTATCACCTGTATCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAGCTGGAAGAACAGGTCCTGAAACTGGTCCATCCCGAACTGTTCCATG
TCGGCAGCTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCCCTCACCGACGTGCTGCTGCTCTGCGGCCTGTTCTACGCCGGCGGCGGCATGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTAGCGATCGCCAACATCCTCCTGCGCGGGCGCATCGGACTGGTGATCGCGGCGGTGGCCAGCCTGG
GCCTGCTGTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCCCCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAACAGACGGAAACGCTGGCCGAGGAACG
CGCCGAGACGGTCGCCAACCTCGAAGAGCTCAACGCGCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGGTCGACA
GCCACCTGAGCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACCAGGTGCGGGGCGCCAGCCTGGAC
CGCTACAGCCCGATGCTGGTGCACTGCATGAAGCAGTGGCGCCTCAACCCCAGTCGCCGCCCGCCGACCCTCAAGGTGAT
TCCGGAGGGCCCCGCCGTGCAGCCCAGCTTCATCAGCCTCAACCGTGAACACGACCGGCATGTGCTGATCTTCCTCGAAG
ACATCTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTCGCCGGCCTCGGCCGGCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCCATCAGCCACGCCGCGCAACTGCTGCAGGAGTCCGAGGAACTCGACGGCGCCGACCGCCG
CCTGACACAGATCATCCAGGACCAGTCGCGACGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGCCGCCGCC
AGGCCGAACCGCAGCAACTCGACCTGAAGGACTGGCTGCAACGCTTCGTCGACGAATATCCCGGCAAGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGCGCCGGCGACATCCACACGCGCATGGACCCGCACCAGTTGAATCAGGTACTGAGCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGTCTCGCGCGCGATGCGG
ACAGCGACCTGCCGGTGCTGGAAATCGCCGACGACGGCCCCGGCGTGCCGACGGACAAGCTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAATTGTGCGAGAGCAACCAGGCGCGCAT
CGACTACCGCAATCGCGAGGAGGGTGGAGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3IWC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

94.528

100

0.945

Multiple sequence alignment