Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   EJN89_RS00215 Genome accession   NZ_CP034441
Coordinates   44618..46606 (-) Length   662 a.a.
NCBI ID   WP_000481443.1    Uniprot ID   P66962
Organism   Staphylococcus aureus strain PMB 81-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 39618..51606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN89_RS00195 sbcD 42207..43328 (-) 1122 WP_000691284.1 exonuclease subunit SbcD -
  EJN89_RS00200 - 43452..43919 (-) 468 WP_000966142.1 CcdC family protein -
  EJN89_RS00205 - 44013..44111 (-) 99 WP_001789897.1 hypothetical protein -
  EJN89_RS00210 - 44098..44340 (-) 243 WP_000246909.1 YneF family protein -
  EJN89_RS00215 recP/tkt 44618..46606 (-) 1989 WP_000481443.1 transketolase Machinery gene
  EJN89_RS00220 - 46727..46966 (-) 240 WP_000071351.1 DUF896 domain-containing protein -
  EJN89_RS00225 sosA 47102..47335 (-) 234 WP_000499647.1 DNA damage-induced cell division inhibitor SosA -
  EJN89_RS00230 dinR/lexA 47478..48101 (+) 624 WP_001208760.1 transcriptional repressor LexA Regulator
  EJN89_RS00235 - 48481..49497 (-) 1017 WP_168989892.1 CAP domain-containing protein -
  EJN89_RS00245 guaC 49519..50495 (-) 977 Protein_43 GMP reductase -
  EJN89_RS00250 rpsN 50652..50921 (-) 270 WP_001085655.1 30S ribosomal protein S14 -
  EJN89_RS00255 rpmG 51375..51524 (-) 150 WP_001265708.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 662 a.a.        Molecular weight: 72250.84 Da        Isoelectric Point: 4.7072

>NTDB_id=289630 EJN89_RS00215 WP_000481443.1 44618..46606(-) (recP/tkt) [Staphylococcus aureus strain PMB 81-4]
MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWTRHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVS
GSLELEELKQFRQWGSKTPGHPEYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKDGNDLEEIDKAITTAKSQEGPTI
IEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFENYGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAET
YPELAEEFKLAISGKLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSETPE
GKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIMGLNATFIFTHDSIAVGEDGPTHEPIEQLAG
LRAIPNMNVIRPADGNETRVAWEVALESESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSE
VSLAVEAAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKVIAIDGFGASAP
GDLVVEKYGFTKENILNQVMSL

Nucleotide


Download         Length: 1989 bp        

>NTDB_id=289630 EJN89_RS00215 WP_000481443.1 44618..46606(-) (recP/tkt) [Staphylococcus aureus strain PMB 81-4]
ATGTTTAATGAAAAAGATCAATTAGCTGTTGATACGCTACGTGCACTAAGTATCGACACAATCGAAAAAGCGAATTCTGG
TCATCCAGGATTACCTATGGGAGCTGCCCCAATGGCTTACACTTTGTGGACACGTCATCTGAATTTTAATCCACAATCTA
AAGATTACTTCAATAGAGACCGTTTCGTATTATCTGCAGGGCATGGTTCAGCATTATTGTATAGCTTGTTACATGTTTCT
GGTAGTTTAGAATTAGAAGAATTAAAGCAATTTAGACAATGGGGTTCTAAAACACCAGGTCATCCTGAATACAGACATAC
AGATGGTGTAGAAGTTACTACCGGACCACTTGGACAAGGTTTTGCTATGTCAGTAGGATTAGCTTTAGCAGAAGATCACC
TAGCAGGGAAATTTAATAAAGAAGGATATAATGTTGTAGATCATTACACATATGTATTAGCTTCTGACGGTGATTTAATG
GAAGGTATATCGCATGAAGCAGCTTCATTTGCTGGACATAATAAATTAAGTAAATTAGTTGTTTTATACGATTCAAATGA
TATTTCATTAGATGGCGAATTAAACAAAGCTTTTTCTGAAAACACAAAAGCTCGTTTTGAAGCATATGGTTGGAATTACT
TACTAGTTAAAGATGGTAATGATTTAGAAGAAATTGATAAAGCGATTACTACAGCTAAATCTCAAGAAGGACCAACGATT
ATTGAAGTTAAAACAACAATCGGATTTGGTTCACCGAATAAAGCAGGAACTAATGGTGTTCATGGGGCACCTTTAGGTGA
AGTTGAAAGAAAATTAACATTCGAAAATTACGGTTTAGATCCTGAAAAACGTTTTAATGTTTCAGAAGAGGTATACGAAA
TTTTCCAAAATACTATGTTAAAACGTGCTAATGAAGATGAATCTCAATGGAATTCATTATTAGAAAAATATGCAGAAACA
TATCCTGAATTAGCAGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTT
TGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCT
TTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTAAATGATGCAACAGATTATAGTTCTGAAACACCTGAA
GGTAAAAATGTGTGGTTTGGTGTACGTGAATTTGCTATGGGTGCTGCTGTAAATGGTATGGCTGCACATGGAGGTTTACA
TCCATATGGTGCAACATTCTTCGTATTTAGTGATTATTTAAAACCAGCGTTACGTTTATCATCAATTATGGGATTAAATG
CAACGTTCATCTTCACACATGATTCAATTGCAGTAGGTGAAGATGGTCCTACTCATGAACCAATTGAACAATTAGCTGGA
TTAAGAGCTATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGA
ATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATGTACCAGAAGATGTAGTTGAAG
AAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAA
GTTAGTCTTGCAGTTGAAGCTGCTAAAGATCTTGAAAAACAAGGTAAATCAGTGCGTGTTGTTTCAATGCCTAACTGGAA
TGCATTTGAACAACAATCTGAAGAATATAAAGAATCAGTTATTCCATCAAGCGTAACAAAACGTGTTGCGATTGAAATGG
CTTCACCGCTTGGATGGCATAAATATGTAGGTACTGCAGGTAAAGTTATTGCTATTGACGGCTTTGGCGCAAGTGCACCT
GGCGATTTAGTAGTTGAAAAATATGGATTTACTAAAGAAAATATCTTAAACCAAGTTATGAGCTTATAA

Domains

Predicted by InterProScan.

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(6-337)

Transketolase, C-terminal domain (Transketolase_C)

(548-655)

Transketolase, pyrimidine binding domain (Transket_pyr)

(359-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P66962

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

55.105

100

0.554