Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   APPSER1_RS06905 Genome accession   NZ_CP029003
Coordinates   1498070..1498618 (-) Length   182 a.a.
NCBI ID   WP_005598298.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 1 str. 4074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1493070..1503618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APPSER1_RS06890 (APPSER1_06890) - 1493554..1494075 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  APPSER1_RS06895 (APPSER1_06895) - 1494180..1495466 (+) 1287 WP_005598292.1 NAD(P)/FAD-dependent oxidoreductase -
  APPSER1_RS06900 (APPSER1_06900) - 1495656..1497995 (-) 2340 WP_005598295.1 Tex family protein -
  APPSER1_RS06905 (APPSER1_06905) pilF 1498070..1498618 (-) 549 WP_005598298.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  APPSER1_RS06910 (APPSER1_06910) - 1498672..1499853 (-) 1182 WP_005598300.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  APPSER1_RS06935 (APPSER1_06935) gltX 1500757..1502196 (+) 1440 WP_005598316.1 glutamate--tRNA ligase -
  APPSER1_RS06945 (APPSER1_06945) yefM 1502533..1502784 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  APPSER1_RS06950 (APPSER1_06950) - 1502781..1503041 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  APPSER1_RS06955 (APPSER1_06955) - 1503200..1503367 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20641.32 Da        Isoelectric Point: 6.9479

>NTDB_id=289210 APPSER1_RS06905 WP_005598298.1 1498070..1498618(-) (pilF) [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQITFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=289210 APPSER1_RS06905 WP_005598298.1 1498070..1498618(-) (pilF) [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTATTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAATTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGGATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAAGCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.199

93.956

0.434

Multiple sequence alignment