Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DCN14_RS29540 Genome accession   NZ_CP028963
Coordinates   3170141..3171646 (+) Length   501 a.a.
NCBI ID   WP_096507359.1    Uniprot ID   A0A2A4C7Q8
Organism   Burkholderia sp. IDO3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3165141..3176646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCN14_RS29515 (DCN14_29515) kdsD 3166375..3167358 (+) 984 WP_096507354.1 arabinose 5-phosphate isomerase KdsD -
  DCN14_RS29520 (DCN14_29520) - 3167358..3167894 (+) 537 WP_096507355.1 HAD family hydrolase -
  DCN14_RS29525 (DCN14_29525) lptC 3167898..3168512 (+) 615 WP_096507356.1 LPS export ABC transporter periplasmic protein LptC -
  DCN14_RS29530 (DCN14_29530) lptA 3168533..3169198 (+) 666 WP_096507357.1 lipopolysaccharide transport periplasmic protein LptA -
  DCN14_RS29535 (DCN14_29535) lptB 3169195..3169971 (+) 777 WP_096507358.1 LPS export ABC transporter ATP-binding protein -
  DCN14_RS29540 (DCN14_29540) rpoN/rpoN1 3170141..3171646 (+) 1506 WP_096507359.1 RNA polymerase factor sigma-54 Machinery gene
  DCN14_RS29545 (DCN14_29545) raiA 3171806..3172165 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  DCN14_RS29550 (DCN14_29550) - 3172361..3172873 (+) 513 WP_059451610.1 PTS sugar transporter subunit IIA -
  DCN14_RS29555 (DCN14_29555) hprK 3173003..3173971 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  DCN14_RS29560 (DCN14_29560) rapZ 3174047..3174955 (+) 909 WP_096507360.1 RNase adapter RapZ -
  DCN14_RS29565 (DCN14_29565) - 3174982..3175617 (+) 636 WP_096507361.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54775.90 Da        Isoelectric Point: 4.8767

>NTDB_id=289043 DCN14_RS29540 WP_096507359.1 3170141..3171646(+) (rpoN/rpoN1) [Burkholderia sp. IDO3]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQSTEPAA
ANGSSQSGERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLADLPAELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKSGQSWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=289043 DCN14_RS29540 WP_096507359.1 3170141..3171646(+) (rpoN/rpoN1) [Burkholderia sp. IDO3]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATTTGGCGCTGACGCCGCAGCTACAGCAGTCGATCCGGCTTCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAAAACGACGATGAATGGA
TCGCGAGTCCGCTGCGTGTCGCGGCGGATGGATCGCTGATCGCGCAGACGCCGCCCGCGCAAAGCACCGAACCTGCCGCC
GCGAACGGCAGCAGCCAGTCGGGCGAGCGCGCCGAACGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTACGCATC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAGTTCGGCCGCTCGAGCGGCGCATCGGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCATCTCTCCGCGCAACTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
CGCCGATTTGCCAGCCGAACTCGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCCG
CCGGGGTCGGTGCGCGCTCGGCATCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTCGAAATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGATTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCGGGCGCCGCCTACGGCAAGGCCGAAG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGTCCGGCCAGAGCTGGCTTGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCTTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTCGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695

Multiple sequence alignment