Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DBK22_RS17010 Genome accession   NZ_CP028961
Coordinates   3438825..3439262 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM102752     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3433825..3444262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBK22_RS16985 (DBK22_03413) - 3434844..3435980 (+) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -
  DBK22_RS16990 (DBK22_03414) - 3435995..3436429 (+) 435 WP_007409358.1 RDD family protein -
  DBK22_RS16995 (DBK22_03415) - 3436547..3436825 (+) 279 WP_372691166.1 YckD family protein -
  DBK22_RS17000 (DBK22_03416) - 3436929..3438365 (+) 1437 WP_098080961.1 glycoside hydrolase family 1 protein -
  DBK22_RS17005 (DBK22_03417) nin/comJ 3438406..3438804 (-) 399 WP_060563061.1 competence protein ComJ Regulator
  DBK22_RS17010 (DBK22_03418) nucA/comI 3438825..3439262 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DBK22_RS17015 (DBK22_03419) hxlB 3439619..3440176 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  DBK22_RS17020 (DBK22_03420) hxlA 3440173..3440808 (-) 636 WP_012116769.1 3-hexulose-6-phosphate synthase -
  DBK22_RS17025 (DBK22_03421) - 3441040..3441402 (+) 363 WP_012116770.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=289025 DBK22_RS17010 WP_003156588.1 3438825..3439262(-) (nucA/comI) [Bacillus velezensis strain SRCM102752]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=289025 DBK22_RS17010 WP_003156588.1 3438825..3439262(-) (nucA/comI) [Bacillus velezensis strain SRCM102752]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACAAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment