Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM371_RS03585 Genome accession   NZ_CP028946
Coordinates   755290..756312 (-) Length   340 a.a.
NCBI ID   WP_039567206.1    Uniprot ID   -
Organism   Serratia marcescens strain AR_0124     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 750290..761312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM371_RS03555 (AM371_03555) - 751094..751606 (+) 513 WP_033649592.1 SprT family zinc-dependent metalloprotease -
  AM371_RS03560 (AM371_03560) endA 751708..752403 (+) 696 WP_033649591.1 deoxyribonuclease I -
  AM371_RS03565 (AM371_03565) rsmE 752473..753204 (+) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM371_RS03570 (AM371_03570) gshB 753215..754165 (+) 951 WP_033649589.1 glutathione synthase -
  AM371_RS03575 (AM371_03575) - 754308..754871 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  AM371_RS03580 (AM371_03580) ruvX 754871..755293 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AM371_RS03585 (AM371_03585) pilT 755290..756312 (-) 1023 WP_039567206.1 type IV pilus twitching motility protein PilT Machinery gene
  AM371_RS03590 (AM371_03590) - 756333..757040 (+) 708 WP_060426992.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM371_RS03595 (AM371_03595) proC 757060..757881 (+) 822 WP_033649586.1 pyrroline-5-carboxylate reductase -
  AM371_RS03600 (AM371_03600) - 757913..758467 (+) 555 WP_033635944.1 YggT family protein -
  AM371_RS03605 (AM371_03605) yggU 758464..758754 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  AM371_RS03610 (AM371_03610) - 758772..759365 (+) 594 WP_060388227.1 XTP/dITP diphosphatase -
  AM371_RS03615 (AM371_03615) hemW 759358..760500 (+) 1143 WP_060388228.1 radical SAM family heme chaperone HemW -
  AM371_RS24925 (AM371_03620) - 760537..760623 (-) 87 Protein_705 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36416.66 Da        Isoelectric Point: 6.8881

>NTDB_id=288763 AM371_RS03585 WP_039567206.1 755290..756312(-) (pilT) [Serratia marcescens strain AR_0124]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=288763 AM371_RS03585 WP_039567206.1 755290..756312(-) (pilT) [Serratia marcescens strain AR_0124]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACCGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGTTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGTGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

50.149

98.529

0.494
  pilT Neisseria meningitidis 8013

50.755

97.353

0.494
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment