Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DB278_RS06725 Genome accession   NZ_CP028926
Coordinates   1382132..1382677 (+) Length   181 a.a.
NCBI ID   WP_108511785.1    Uniprot ID   -
Organism   Pasteurella multocida strain 20N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1377132..1387677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB278_RS06700 (DB278_06700) - 1377557..1378486 (+) 930 WP_059245741.1 phosphatidate cytidylyltransferase -
  DB278_RS06705 (DB278_06705) mog 1378512..1379090 (+) 579 WP_108511784.1 molybdopterin adenylyltransferase -
  DB278_RS06710 (DB278_06710) glnB 1379135..1379473 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  DB278_RS06715 (DB278_06715) - 1379474..1380727 (+) 1254 WP_005755688.1 AI-2E family transporter -
  DB278_RS06720 (DB278_06720) - 1380908..1382092 (+) 1185 WP_083004640.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DB278_RS06725 (DB278_06725) pilF/pilF2 1382132..1382677 (+) 546 WP_108511785.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DB278_RS06730 (DB278_06730) - 1382800..1383753 (+) 954 WP_083004644.1 helix-turn-helix domain-containing protein -
  DB278_RS06735 (DB278_06735) ispG 1383780..1384883 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DB278_RS06740 (DB278_06740) hisS 1384913..1386184 (+) 1272 WP_108511786.1 histidine--tRNA ligase -
  DB278_RS06745 (DB278_06745) - 1386203..1386814 (+) 612 WP_083004649.1 YfgM family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20833.40 Da        Isoelectric Point: 7.9057

>NTDB_id=288617 DB278_RS06725 WP_108511785.1 1382132..1382677(+) (pilF/pilF2) [Pasteurella multocida strain 20N]
MTRVFQLGIASIFSLFLSACVSQEGHKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFEPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=288617 DB278_RS06725 WP_108511785.1 1382132..1382677(+) (pilF/pilF2) [Pasteurella multocida strain 20N]
ATGACGCGCGTTTTTCAATTAGGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTCACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACACTTAACGCAGTTTGAGCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453

Multiple sequence alignment