Detailed information NTG0288569

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EHC69_RS01375
• Genome accession: NZ_CP034298
• Coordinates: 273961..276165 (-)
• Length: 734 a.a.
• NCBI ID: WP_025789388.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain 20160303005-1
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 268961..281165

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EHC69_RS01350 (EHC69_01365)	-	269179..269403 (+)	225	WP_005489715.1	hypothetical protein	-
EHC69_RS01355 (EHC69_01370)	hslU	269828..271159 (-)	1332	WP_005489452.1	HslU--HslV peptidase ATPase subunit	-
EHC69_RS01360 (EHC69_01375)	hslV	271183..271734 (-)	552	WP_025789387.1	ATP-dependent protease subunit HslV	-
EHC69_RS01365 (EHC69_01380)	ftsN	271899..272444 (-)	546	WP_005481405.1	cell division protein FtsN	-
EHC69_RS01370 (EHC69_01385)	cytR	272621..273628 (-)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
EHC69_RS01375 (EHC69_01390)	priA	273961..276165 (-)	2205	WP_025789388.1	primosomal protein N'	Machinery gene
EHC69_RS01385 (EHC69_01400)	rpmE	276460..276681 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
EHC69_RS01390 (EHC69_01405)	rpsJ	277139..277450 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
EHC69_RS01395 (EHC69_01410)	rplC	277465..278094 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
EHC69_RS01400 (EHC69_01415)	rplD	278112..278714 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
EHC69_RS01405 (EHC69_01420)	rplW	278711..279013 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
EHC69_RS01410 (EHC69_01425)	rplB	279029..279853 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
EHC69_RS01415 (EHC69_01430)	rpsS	279875..280153 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
EHC69_RS01420 (EHC69_01435)	rplV	280164..280496 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82258.83 Da
• Isoelectric Point: 8.6765

>NTDB_id=288569 EHC69_RS01375 WP_025789388.1 273961..276165(-) (priA) [Vibrio parahaemolyticus strain 20160303005-1]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPIWSEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITKWGQHVECDVEKPKLNHEQALAIASVNSQAGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTSNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTNAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=288569 EHC69_RS01375 WP_025789388.1 273961..276165(-) (priA) [Vibrio parahaemolyticus strain 20160303005-1]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGGCGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCAAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGATATGGTCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAAAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCAA
GTGGGGGCAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGTGTAAATA
GCCAAGCAGGTTTTGCCTGCTATTTACTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAA
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTTCTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCGCAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTGGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGAGATAGCACGCGCCGCAAAGGCAGTTTAGAAGATGCGCTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGTTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTACAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAAG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCTCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAATGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (220-365)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.691	100	0.439
priA	Bacillus subtilis subsp. subtilis str. 168	34.005	100	0.368