Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AOY78_RS18235 Genome accession   NZ_CP028750
Coordinates   3741160..3742140 (-) Length   326 a.a.
NCBI ID   WP_085455522.1    Uniprot ID   -
Organism   Escherichia coli strain A25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3736160..3747140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY78_RS18205 (AOY78_19310) yggI 3736825..3737322 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  AOY78_RS18210 (AOY78_19315) endA 3737417..3738124 (+) 708 WP_001327408.1 deoxyribonuclease I -
  AOY78_RS18215 (AOY78_19320) rsmE 3738204..3738935 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AOY78_RS18220 (AOY78_19325) gshB 3738948..3739898 (+) 951 WP_000593259.1 glutathione synthase -
  AOY78_RS18225 (AOY78_19330) yqgE 3740007..3740570 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  AOY78_RS18230 (AOY78_19335) ruvX 3740570..3740986 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  AOY78_RS18235 (AOY78_19340) pilT 3741160..3742140 (-) 981 WP_085455522.1 type IV pilus twitching motility protein PilT Machinery gene
  AOY78_RS18240 (AOY78_19345) yggS 3742158..3742862 (+) 705 WP_000997807.1 pyridoxal phosphate homeostasis protein -
  AOY78_RS18245 (AOY78_19350) yggT 3742880..3743446 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  AOY78_RS18250 (AOY78_19355) yggU 3743443..3743733 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  AOY78_RS18255 (AOY78_19360) rdgB 3743741..3744334 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  AOY78_RS18260 (AOY78_19365) hemW 3744327..3745463 (+) 1137 WP_044706006.1 radical SAM family heme chaperone HemW -
  AOY78_RS18265 (AOY78_19370) yggM 3745528..3746535 (-) 1008 WP_000745232.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.34 Da        Isoelectric Point: 5.9975

>NTDB_id=287239 AOY78_RS18235 WP_085455522.1 3741160..3742140(-) (pilT) [Escherichia coli strain A25]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPLILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=287239 AOY78_RS18235 WP_085455522.1 3741160..3742140(-) (pilT) [Escherichia coli strain A25]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACTGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.906

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment