Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   NGK_RS02770 Genome accession   NC_011035
Coordinates   521295..521906 (+) Length   203 a.a.
NCBI ID   WP_012503481.1    Uniprot ID   B4RKG6
Organism   Neisseria gonorrhoeae NCCP11945     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516295..526906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK_RS02745 (NGK_0620) - 516646..517227 (-) 582 WP_003690895.1 superoxide dismutase -
  NGK_RS02750 (NGK_0622) dnaB 517391..518794 (+) 1404 WP_012503477.1 replicative DNA helicase -
  NGK_RS16520 - 518788..518900 (-) 113 Protein_517 IS5/IS1182 family transposase -
  NGK_RS02755 pilH 519052..519714 (+) 663 WP_041421240.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  NGK_RS02760 (NGK_0624) pilV/pilI 519746..520360 (+) 615 WP_012503479.1 type IV pilus modification protein PilV Regulator
  NGK_RS02765 (NGK_0625) pilJ 520357..521316 (+) 960 WP_012503480.1 PilW family protein Machinery gene
  NGK_RS02770 (NGK_0626) pilK 521295..521906 (+) 612 WP_012503481.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  NGK_RS02775 (NGK_0627) pilL 521908..522381 (+) 474 WP_012503482.1 PilX family type IV pilin Machinery gene
  NGK_RS02780 (NGK_0628) - 522451..522759 (-) 309 WP_003706588.1 AzlD family protein -
  NGK_RS02785 - 522756..523467 (-) 712 Protein_524 AzlC family ABC transporter permease -
  NGK_RS02790 (NGK_0631) dut 523633..524085 (+) 453 WP_003701071.1 dUTP diphosphatase -
  NGK_RS02795 (NGK_0632) dapC 524157..525344 (+) 1188 WP_003701073.1 succinyldiaminopimelate transaminase -
  NGK_RS02800 (NGK_0633) yaaA 525500..526279 (+) 780 WP_003687925.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 22021.91 Da        Isoelectric Point: 8.0862

>NTDB_id=28536 NGK_RS02770 WP_012503481.1 521295..521906(+) (pilK) [Neisseria gonorrhoeae NCCP11945]
MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYAA
DSKVTFSENCEKGLCTAVNVRTNDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGNVSKMP
RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=28536 NGK_RS02770 WP_012503481.1 521295..521906(+) (pilK) [Neisseria gonorrhoeae NCCP11945]
ATGCGCAAACAGAACACTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTCCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGCGGGAGGGCGAATTTCAGGTTTTGGATTTGGAATATGCTGCG
GACAGTAAGGTTACGTTTAGCGAAAACTGTGAAAAAGGTCTGTGTACCGCAGTGAATGTGCGGACAAATGATAATGGTAA
TGAAGAGGCTTTTGGCAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAAGTCTG
GCAAAAATTCTACCGGCCTGTGCATTGACAATCAGGGAGTGGAATATAAGAAAGGTACGGGAAACGTCAGCAAAATGCCG
CGCTATATTATCGAATATTTAGGCGTGAAGAACGGACAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGC
CAATACCGTGGTCGTCCTTCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterProScan.

PilX N-terminal (PilX_N)

(17-67)

Type IV pilus assembly protein PilX C-term (PilX)

(92-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4RKG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

96.059

100

0.961

Multiple sequence alignment