Detailed information NTG0028535

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSPA7_RS02360
• Genome accession: NC_009656
• Coordinates: 504200..505348 (+)
• Length: 382 a.a.
• NCBI ID: WP_033999672.1
• Uniprot ID: A6UYK6
• Organism: Pseudomonas paraeruginosa PA7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 499200..510348

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSPA7_RS02335 (PSPA7_0491)	-	500349..500645 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PSPA7_RS02340 (PSPA7_0492)	-	500642..501235 (-)	594	WP_003155373.1	YggT family protein	-
PSPA7_RS02345 (PSPA7_0493)	proC	501246..502067 (-)	822	WP_012073993.1	pyrroline-5-carboxylate reductase	-
PSPA7_RS02350 (PSPA7_0494)	-	502079..502771 (-)	693	WP_003155374.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSPA7_RS02355 (PSPA7_0495)	pilT	502986..504020 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PSPA7_RS02360 (PSPA7_0496)	pilU	504200..505348 (+)	1149	WP_033999672.1	type IV pilus ATPase PilU	Machinery gene
PSPA7_RS02365 (PSPA7_0497)	czcD	505356..506255 (-)	900	WP_012073995.1	CDF family zinc transporter CzcD	-
PSPA7_RS02370 (PSPA7_0498)	-	506408..506812 (+)	405	WP_003155375.1	NINE protein	-
PSPA7_RS02375 (PSPA7_0499)	-	507054..508427 (+)	1374	WP_003157911.1	pyridoxal-phosphate dependent enzyme	-
PSPA7_RS02380 (PSPA7_0500)	-	508424..509608 (+)	1185	WP_012073996.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42536.81 Da
• Isoelectric Point: 6.7790

>NTDB_id=28535 PSPA7_RS02360 WP_033999672.1 504200..505348(+) (pilU) [Pseudomonas paraeruginosa PA7]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSMTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=28535 PSPA7_RS02360 WP_033999672.1 504200..505348(+) (pilU) [Pseudomonas paraeruginosa PA7]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAGAAAGGCGGCTCCGACCTGTTCATCACCGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGTCGGGTGATGCCGGTGACCAAGACCCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTCGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGCGCCTTTTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGGATCGAGACCAACATTCCCACCCTGGA
CGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAATACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGATACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGTTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCGATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAAGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGGCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCATCTGTCGAGCATGACCCAGGGCCTGAGCCTGGAGATCACCGACG
ACGACCCGGCCGGTCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A6UYK6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.546	93.455	0.613
pilU	Vibrio cholerae strain A1552	60	91.623	0.55
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382