Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ASUC_RS10490 Genome accession   NC_009655
Coordinates   2234893..2235432 (-) Length   179 a.a.
NCBI ID   WP_012073751.1    Uniprot ID   A6VQX8
Organism   Actinobacillus succinogenes 130Z     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2229893..2240432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASUC_RS10470 (Asuc_2025) - 2230776..2231384 (-) 609 WP_012073747.1 tetratricopeptide repeat protein -
  ASUC_RS10475 (Asuc_2026) hisS 2231396..2232667 (-) 1272 WP_012073748.1 histidine--tRNA ligase -
  ASUC_RS10480 (Asuc_2027) ispG 2232740..2233855 (-) 1116 WP_012073749.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ASUC_RS10485 (Asuc_2028) - 2233861..2234799 (-) 939 WP_012073750.1 RodZ family helix-turn-helix domain-containing protein -
  ASUC_RS10490 (Asuc_2029) pilF/pilF2 2234893..2235432 (-) 540 WP_012073751.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ASUC_RS10495 (Asuc_2030) - 2235453..2236568 (-) 1116 WP_012073752.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ASUC_RS10500 (Asuc_2031) ispF 2236856..2237332 (-) 477 WP_012073753.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ASUC_RS10505 (Asuc_2032) ispD 2237342..2238055 (-) 714 WP_012073754.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ASUC_RS10510 (Asuc_2033) ftsB 2238055..2238333 (-) 279 WP_012073755.1 cell division protein FtsB -
  ASUC_RS10515 (Asuc_2034) - 2238451..2239068 (-) 618 WP_012073756.1 NapC/NirT family cytochrome c -
  ASUC_RS10520 (Asuc_2035) - 2239080..2239508 (-) 429 WP_041834825.1 nitrate reductase cytochrome c-type subunit -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20399.06 Da        Isoelectric Point: 6.6263

>NTDB_id=28532 ASUC_RS10490 WP_012073751.1 2234893..2235432(-) (pilF/pilF2) [Actinobacillus succinogenes 130Z]
MKLCKFTPHFLFSLFLCACSSLPDEDFDKQQAAKARVELALGYLAQNNPMQAKLNLDKALNYAPDYYLVHSALAYFHHQQ
GNIELADNAYRKAISLDDDQGDVHNNYGTFLCSLGKAEQGIEQFRKALSAKYYYNQTDTYENLAICAYSVKNRAVYQESI
DLLRKISPTRAAELESLRF

Nucleotide


Download         Length: 540 bp        

>NTDB_id=28532 ASUC_RS10490 WP_012073751.1 2234893..2235432(-) (pilF/pilF2) [Actinobacillus succinogenes 130Z]
ATGAAACTTTGTAAATTTACACCGCATTTTTTGTTCTCCTTGTTTCTTTGCGCCTGCTCATCTTTACCGGATGAAGATTT
TGATAAACAACAAGCGGCTAAAGCTCGGGTTGAATTGGCGTTGGGTTATCTTGCCCAGAATAATCCGATGCAGGCAAAAT
TAAATCTGGACAAGGCCTTAAATTACGCGCCGGATTATTATCTGGTGCATTCTGCACTGGCTTATTTTCATCATCAACAG
GGCAACATCGAGCTGGCGGACAATGCATATCGTAAAGCGATTTCGTTGGATGACGATCAAGGCGACGTACATAATAATTA
CGGTACTTTTTTATGTTCCCTAGGCAAAGCCGAGCAGGGAATCGAACAATTCCGAAAAGCTTTATCCGCCAAGTATTATT
ATAATCAAACGGATACCTACGAGAATCTGGCAATTTGTGCTTATTCCGTGAAAAATCGTGCGGTTTATCAGGAAAGTATC
GACTTATTACGTAAAATCTCACCGACACGAGCCGCGGAACTGGAGTCTCTACGGTTCTGA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(72-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6VQX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

51.786

93.855

0.486
  pilF Glaesserella parasuis strain SC1401

40.936

95.531

0.391

Multiple sequence alignment