Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   DA379_RS09450 Genome accession   NZ_CP028441
Coordinates   1968672..1969472 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain 131-4     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 1963672..1974472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA379_RS09425 - 1964218..1964850 (+) 633 WP_003153189.1 helix-turn-helix domain-containing protein -
  DA379_RS19645 - 1965141..1965278 (+) 138 WP_014470646.1 hypothetical protein -
  DA379_RS09430 - 1965372..1966490 (+) 1119 WP_003153184.1 NADH-dependent flavin oxidoreductase -
  DA379_RS09435 - 1966617..1966829 (+) 213 WP_003153183.1 spore germination protein -
  DA379_RS09440 - 1966955..1967167 (+) 213 WP_003153181.1 spore germination protein -
  DA379_RS09445 alr 1967343..1968524 (+) 1182 WP_003153179.1 alanine racemase -
  DA379_RS09450 spo0A 1968672..1969472 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  DA379_RS09455 spoIVB 1969753..1971033 (-) 1281 WP_003153176.1 SpoIVB peptidase -
  DA379_RS09460 recN 1971197..1972927 (-) 1731 WP_003153174.1 DNA repair protein RecN Machinery gene
  DA379_RS09465 argR 1972959..1973408 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  DA379_RS09470 - 1973503..1974348 (-) 846 WP_003153172.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=285073 DA379_RS09450 WP_003153177.1 1968672..1969472(-) (spo0A) [Bacillus velezensis strain 131-4]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=285073 DA379_RS09450 WP_003153177.1 1968672..1969472(-) (spo0A) [Bacillus velezensis strain 131-4]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGTG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGGTATACCGTAAGC
ATGACAAAAGCAAAACCAACCAACTCGGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment