Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SK637_RS00140 Genome accession   NZ_CP028415
Coordinates   25197..26561 (+) Length   454 a.a.
NCBI ID   WP_135783528.1    Uniprot ID   -
Organism   Streptococcus mitis strain SK637     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20197..31561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK637_RS00095 (SK637_00020) comW 20591..20827 (+) 237 WP_033687874.1 sigma(X)-activator ComW Regulator
  SK637_RS00100 (SK637_00021) - 21058..22344 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  SK637_RS00105 (SK637_00022) tadA 22544..23011 (+) 468 WP_033687875.1 tRNA adenosine(34) deaminase TadA -
  SK637_RS00115 (SK637_00023) - 23198..23641 (+) 444 WP_000701983.1 dUTP diphosphatase -
  SK637_RS00120 (SK637_00024) - 23643..24182 (+) 540 WP_033687876.1 histidine phosphatase family protein -
  SK637_RS00125 - 24193..24354 (+) 162 Protein_19 DNA repair protein RadA -
  SK637_RS00130 (SK637_00025) - 24350..24652 (+) 303 Protein_20 isochorismatase family protein -
  SK637_RS00135 (SK637_00026) - 24671..25186 (+) 516 WP_033687877.1 histidine phosphatase family protein -
  SK637_RS00140 (SK637_00027) radA 25197..26561 (+) 1365 WP_135783528.1 DNA repair protein RadA Machinery gene
  SK637_RS00145 (SK637_00028) - 26634..27131 (+) 498 WP_033687878.1 beta-class carbonic anhydrase -
  SK637_RS00150 (SK637_00029) - 27156..27971 (+) 816 WP_033687879.1 PrsW family intramembrane metalloprotease -
  SK637_RS00155 (SK637_00030) - 28117..29085 (+) 969 WP_033682853.1 ribose-phosphate diphosphokinase -
  SK637_RS00160 (SK637_00031) - 29204..30175 (+) 972 WP_033687880.1 Rpn family recombination-promoting nuclease/putative transposase -
  SK637_RS09925 (SK637_00032) - 30190..30664 (+) 475 Protein_27 hypothetical protein -
  SK637_RS00170 (SK637_00033) - 30679..31032 (+) 354 Protein_28 hypothetical protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49530.07 Da        Isoelectric Point: 6.5137

>NTDB_id=284670 SK637_RS00140 WP_135783528.1 25197..26561(+) (radA) [Streptococcus mitis strain SK637]
MIAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEV
ERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGE
RHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNA
KRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEI
RRVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=284670 SK637_RS00140 WP_135783528.1 25197..26561(+) (radA) [Streptococcus mitis strain SK637]
ATCATAGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCCAA
CTGTGGGTCTTGGTCTTCTTTTGTGGAAGAAGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGA
AAACCAAGCCCATGAAACTGGCTGAGGTGACTTCAATCAACGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGT
GTACTCGGTGGGGGTGTGGTACCAGGGAGTCTCGTCCTTATCGGTGGGGATCCTGGAATCGGGAAATCAACCCTTCTCCT
ACAAGTATCAACCCAGTTGTCTCAAGTGGGGACTGTTCTCTACGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAGCTAC
GAGCAGAGCGCTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCCGAGACCAATATGCAGAGTGTTCGAGCAGAGGTG
GAGCGCATCCAACCAGACTTCCTCATTATTGACTCTATCCAGACCATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTC
TGTTTCTCAAGTGCGTGAGGTGACGGCTGAGCTCATGCAACTGGCCAAGACTAATAACATCGCCATCTTTATCGTAGGTC
ATGTGACCAAGGAAGGAACCTTGGCTGGTCCTCGTATGCTAGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAG
CGCCACCATACCTTCCGTATTTTAAGAGCGGTCAAAAACCGTTTTGGTTCTACCAATGAGATTGGGATTTTTGAGATGCA
GTCGGGCGGATTGGTTGAGGTGCTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGACGGGGCAACTGGTTCGTCAA
TCGTTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTGACACCTACCATGTTTGGAAATGCC
AAGCGTACGACGACGGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGTCTTCTCTT
GCAAAATCAGGATGCTTATCTTAAATCTGCTGGCGGTGTCAAATTGGATGAACCTGCAATAGACTTGGCCGTTGCGGTTG
CTATTGCTTCGAGCTATAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTGGGAGAACTGGGCTTGACAGGAGAGATT
CGGCGTGTGAATCGTATCGAACAACGCATCAATGAAGCTGCTAAACTGGGATTTACTAAGATTTATGTACCTAAAAATTC
CTTGACAGGAATTACTCCACCCAAGGAAATTCAGGTCATTGGAGTAACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTG
CATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-219)

Subunit ChlI of Mg-chelatase (ChlI)

(361-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.558

99.78

0.993
  radA Streptococcus mitis SK321

99.558

99.78

0.993
  radA Streptococcus pneumoniae Rx1

99.558

99.78

0.993
  radA Streptococcus pneumoniae D39

99.558

99.78

0.993
  radA Streptococcus pneumoniae R6

99.558

99.78

0.993
  radA Streptococcus pneumoniae TIGR4

99.558

99.78

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

62.914

99.78

0.628

Multiple sequence alignment