Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Tharo_RS15020 Genome accession   NZ_CP028339
Coordinates   3170060..3171478 (+) Length   472 a.a.
NCBI ID   WP_107221903.1    Uniprot ID   A0A2R4BRD7
Organism   Thauera aromatica K172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3165060..3176478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tharo_RS14990 (Tharo_2963) thiL 3165283..3166284 (+) 1002 WP_107221897.1 thiamine-phosphate kinase -
  Tharo_RS14995 (Tharo_2964) - 3166559..3167041 (+) 483 WP_107221898.1 phosphatidylglycerophosphatase A -
  Tharo_RS15000 (Tharo_2965) - 3167094..3167582 (+) 489 WP_107221899.1 nicotinamide-nucleotide amidohydrolase family protein -
  Tharo_RS15005 (Tharo_2966) recA 3167831..3168865 (+) 1035 WP_107221900.1 recombinase RecA Machinery gene
  Tharo_RS15010 (Tharo_2967) - 3168882..3169331 (+) 450 WP_107221901.1 regulatory protein RecX -
  Tharo_RS15015 (Tharo_2968) lptB 3169318..3170043 (+) 726 WP_107221902.1 LPS export ABC transporter ATP-binding protein -
  Tharo_RS15020 (Tharo_2969) rpoN/rpoN1 3170060..3171478 (+) 1419 WP_107221903.1 RNA polymerase factor sigma-54 Machinery gene
  Tharo_RS15025 (Tharo_2970) raiA 3171496..3171819 (+) 324 WP_075147869.1 ribosome-associated translation inhibitor RaiA -
  Tharo_RS15030 (Tharo_2971) ptsN 3171956..3172441 (+) 486 WP_107221904.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Tharo_RS15035 (Tharo_2972) hprK 3172410..3173357 (+) 948 WP_107221905.1 HPr(Ser) kinase/phosphatase -
  Tharo_RS15040 (Tharo_2973) ilvA 3173404..3175038 (+) 1635 WP_281257407.1 threonine ammonia-lyase, biosynthetic -
  Tharo_RS15045 (Tharo_2974) - 3175113..3175733 (-) 621 WP_245880920.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 52365.17 Da        Isoelectric Point: 5.0845

>NTDB_id=284230 Tharo_RS15020 WP_107221903.1 3170060..3171478(+) (rpoN/rpoN1) [Thauera aromatica K172]
MKTSLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERFLLENPMLEREDDEGGDAPPPPPGAERDAPPAEGEADARTH
DGAEDEGIDWSNVGQGAGSRSEDDDSDYQDLQAADISLREHLDQQIALSPLSDRDRALARLIIEALDDDGYLHPPLEELL
ELLAPESGVTLDDLAIALHHVQHLDPAGIAARTPQECLSLQLRALPEDGTRALALEIVDKHLALLAERNFARLKKLTGCD
DDGLRAAQALICSLDPHPGTRYGLHETRYVLPDVVVRKVRGHWTVRLNQEAMPRLRINRLYASLLQQNRGQGGGLGAQLQ
EARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVFELKYFFG
SHVATDTGGAASSTAIRALIRQLVDAEDSKKPLSDARIAEVLGQQGIVVARRTIAKYRESLNIPPVSLRKSI

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=284230 Tharo_RS15020 WP_107221903.1 3170060..3171478(+) (rpoN/rpoN1) [Thauera aromatica K172]
ATGAAAACCAGCCTCCAGCTCAAGCTCTCCCAACACCTCACGCTGACCCCACAGCTGCAGCAGTCGATCAAGCTGTTGCA
GCTGTCGACGATCGAGCTGAACCAGGAAATCGAGCGCTTCCTGCTCGAGAACCCGATGCTCGAACGCGAGGACGACGAAG
GCGGAGATGCTCCCCCGCCCCCGCCCGGAGCCGAGCGCGACGCTCCGCCCGCCGAGGGCGAGGCCGACGCACGCACGCAC
GACGGCGCCGAGGACGAAGGCATCGACTGGTCGAACGTCGGCCAGGGCGCCGGCAGCCGCAGCGAAGACGACGATTCCGA
CTACCAGGATCTCCAGGCCGCCGATATCTCGCTGCGCGAGCACCTCGACCAGCAGATCGCCCTGTCCCCGCTGTCGGACC
GCGACCGGGCACTGGCGCGGCTGATCATCGAGGCTCTGGACGACGACGGCTACCTGCACCCGCCGCTGGAAGAACTCCTC
GAACTGCTCGCCCCCGAGTCCGGCGTCACGCTCGACGATCTGGCGATCGCGCTGCATCACGTCCAGCACCTCGATCCGGC
CGGCATCGCCGCGCGCACGCCGCAGGAATGCTTGTCGCTGCAGCTGCGCGCCCTGCCCGAGGACGGCACCCGGGCACTGG
CGCTGGAGATCGTCGACAAGCACCTCGCACTGCTCGCCGAACGCAACTTCGCCCGGCTGAAGAAGCTCACCGGCTGCGAC
GACGACGGCCTGCGTGCCGCCCAGGCCCTGATCTGCAGTCTCGACCCGCATCCGGGCACACGCTACGGCCTGCACGAGAC
CCGCTACGTGCTGCCCGACGTCGTCGTGCGCAAGGTCCGCGGCCACTGGACGGTGCGCCTCAACCAGGAGGCCATGCCGC
GCCTGCGCATCAACCGCCTGTACGCCAGCCTGCTGCAGCAGAACCGCGGCCAGGGCGGCGGGCTGGGCGCCCAGCTGCAG
GAGGCGCGCTGGCTGATCAAGAACGTACAGCAGCGTTTCGACACCATCCTGCGCGTTTCCCAGGCCATCGTTGACCAGCA
GCGGCAGTTCTTCGATCATGGAGACGTGGCGATGCGGCCACTCACTCTCCGGGAGATCGCGGACCAGCTCGAGCTGCACG
AATCCACCGTATCGCGCGTCACCACGCAGAAATTCATGGCCACGCCGCGCGGCGTATTCGAACTCAAGTATTTCTTCGGC
AGCCACGTCGCCACCGACACCGGTGGAGCGGCATCATCAACGGCGATTCGTGCGCTGATTCGCCAGCTGGTCGATGCCGA
AGACAGCAAGAAACCGCTATCCGACGCCAGGATTGCAGAAGTTTTAGGCCAGCAGGGTATCGTGGTCGCACGGCGGACGA
TCGCCAAGTACCGCGAATCGCTGAACATCCCGCCGGTCAGTCTGCGCAAGTCCATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-470)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-301)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R4BRD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.378

100

0.589

Multiple sequence alignment