Detailed information NTG0281903

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: C9J77_RS08975
• Genome accession: NZ_CP028163
• Coordinates: 1702561..1703058 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain CFSAN064038
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1697561..1708058

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C9J77_RS08935 (C9J77_08930)	-	1697605..1698591 (+)	987	WP_000161315.1	ROK family glucokinase	-
C9J77_RS08940 (C9J77_08935)	-	1698591..1698920 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
C9J77_RS08945 (C9J77_08940)	-	1698917..1699540 (+)	624	WP_021285938.1	MBL fold metallo-hydrolase	-
C9J77_RS08950 (C9J77_08945)	comGA	1699592..1700566 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
C9J77_RS08955 (C9J77_08950)	comGB	1700538..1701608 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
C9J77_RS08960 (C9J77_08955)	comGC	1701622..1701933 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
C9J77_RS08965 (C9J77_08960)	comGD	1701911..1702357 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
C9J77_RS08970 (C9J77_08965)	comGE	1702344..1702643 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
C9J77_RS08975 (C9J77_08970)	comGF	1702561..1703058 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
C9J77_RS08980 (C9J77_08975)	-	1703155..1703301 (+)	147	WP_001789879.1	hypothetical protein	-
C9J77_RS08985 (C9J77_08980)	-	1703291..1703815 (+)	525	WP_001015120.1	shikimate kinase	-
C9J77_RS08990 (C9J77_08985)	gcvT	1703974..1705065 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
C9J77_RS08995 (C9J77_08990)	gcvPA	1705085..1706431 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
C9J77_RS09000 (C9J77_08995)	gcvPB	1706424..1707896 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=281903 C9J77_RS08975 WP_001825340.1 1702561..1703058(+) (comGF) [Staphylococcus aureus strain CFSAN064038]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=281903 C9J77_RS08975 WP_001825340.1 1702561..1703058(+) (comGF) [Staphylococcus aureus strain CFSAN064038]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97