Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C9I78_RS01060 Genome accession   NZ_CP028141
Coordinates   210509..211525 (+) Length   338 a.a.
NCBI ID   WP_043036352.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain R14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 205509..216525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9I78_RS01045 (C9I78_01045) - 205933..206661 (-) 729 WP_057619621.1 redoxin family protein -
  C9I78_RS01050 (C9I78_01050) oxyR 206832..207740 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  C9I78_RS01055 (C9I78_01055) - 207796..210366 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  C9I78_RS01060 (C9I78_01060) pilM 210509..211525 (+) 1017 WP_043036352.1 type IV pilus biogenesis protein PilM Machinery gene
  C9I78_RS01065 (C9I78_01065) pilN 211509..212087 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  C9I78_RS01070 (C9I78_01070) pilO 212080..212673 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  C9I78_RS01075 (C9I78_01075) pilP 212663..213178 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  C9I78_RS01080 (C9I78_01080) pilQ 213210..214958 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  C9I78_RS01085 (C9I78_01085) aroK 215147..215665 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37693.17 Da        Isoelectric Point: 6.6634

>NTDB_id=281618 C9I78_RS01060 WP_043036352.1 210509..211525(+) (pilM) [Vibrio parahaemolyticus strain R14]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
SQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=281618 C9I78_RS01060 WP_043036352.1 210509..211525(+) (pilM) [Vibrio parahaemolyticus strain R14]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATTTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
AGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment