Detailed information NTG0280077

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C4K05_RS27015
• Genome accession: NZ_CP027752
• Coordinates: 5982372..5983244 (+)
• Length: 290 a.a.
• NCBI ID: WP_009050909.1
• Uniprot ID: A0AB33WWI2
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR38
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5977372..5988244

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4K05_RS27000 (C4K05_5486)	-	5979727..5980083 (-)	357	WP_009050906.1	BON domain-containing protein	-
C4K05_RS27005 (C4K05_5487)	-	5980371..5980787 (-)	417	WP_009050907.1	pilin	-
C4K05_RS27010 (C4K05_5488)	pilC	5981152..5982369 (+)	1218	WP_028683416.1	type II secretion system F family protein	Machinery gene
C4K05_RS27015 (C4K05_5489)	pilD	5982372..5983244 (+)	873	WP_009050909.1	prepilin peptidase	Machinery gene
C4K05_RS27020 (C4K05_5490)	coaE	5983241..5983864 (+)	624	WP_009045669.1	dephospho-CoA kinase	-
C4K05_RS27025 (C4K05_5491)	yacG	5983861..5984064 (+)	204	WP_009045670.1	DNA gyrase inhibitor YacG	-
C4K05_RS27030 (C4K05_5492)	-	5984069..5984284 (-)	216	WP_009050911.1	hypothetical protein	-
C4K05_RS27035 (C4K05_5493)	-	5984350..5985039 (-)	690	WP_110177023.1	energy-coupling factor ABC transporter permease	-
C4K05_RS27040 (C4K05_5494)	-	5985036..5985503 (-)	468	WP_009050913.1	hypothetical protein	-
C4K05_RS27045 (C4K05_5495)	-	5985959..5987395 (+)	1437	WP_009050914.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32050.22 Da
• Isoelectric Point: 7.9954

>NTDB_id=280077 C4K05_RS27015 WP_009050909.1 5982372..5983244(+) (pilD) [Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR38]
MSLTEVLASYPLVFVASMLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPVEPAGPTYNLLLPHSQCPHCAHSIR
PWENIPVFSYLLLRGRCSACQAPIGRRYPLVELTCGLASAFVAWHFGFGWQAVLMLLLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGIALLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=280077 C4K05_RS27015 WP_009050909.1 5982372..5983244(+) (pilD) [Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR38]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGATGCTGGTCCTGGGCCTGATTGTCGGCAG
CTTTCTCAATGTGCTGATCTGGCGTCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCCCGTGACATCCTGGGGC
TGCCCGTCGAACCTGCCGGGCCGACCTACAACCTGCTGCTCCCGCATTCGCAGTGCCCTCACTGCGCACATTCGATCCGC
CCCTGGGAAAACATCCCGGTGTTCAGCTACCTGCTGCTGCGCGGACGCTGCTCCGCGTGCCAGGCACCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGTCC
TGATGCTGCTGTTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAGCTGTTGCCCGACGTGCTGGTG
CTGCCGCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTTTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGTGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCCCTGGCGGGGATCGCCCTGCTGCGCCGCAACCGTGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.817	97.931	0.517
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	50.909	94.828	0.483
pilD	Acinetobacter nosocomialis M2	50.182	94.828	0.476
pilD	Neisseria gonorrhoeae MS11	47.794	93.793	0.448