Detailed information NTG0027894

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ASA_RS02175
• Genome accession: NC_009348
• Coordinates: 419990..420865 (-)
• Length: 291 a.a.
• NCBI ID: WP_005314162.1
• Uniprot ID: A2T195
• Organism: Aeromonas salmonicida subsp. salmonicida A449
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 414990..425865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ASA_RS02150 (ASA_0406)	-	416727..417899 (+)	1173	WP_005314169.1	tetratricopeptide repeat protein	-
ASA_RS02155 (ASA_0407)	mutT	417908..418315 (+)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
ASA_RS02160 (ASA_0408)	yacG	418392..418586 (-)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
ASA_RS02165 (ASA_0409)	zapD	418596..419318 (-)	723	WP_005314166.1	cell division protein ZapD	-
ASA_RS02170 (ASA_0410)	coaE	419356..419970 (-)	615	WP_005314164.1	dephospho-CoA kinase	-
ASA_RS02175 (ASA_0411)	pilD	419990..420865 (-)	876	WP_005314162.1	A24 family peptidase	Machinery gene
ASA_RS02180 (ASA_0412)	-	420929..422169 (-)	1241	Protein_398	type II secretion system F family protein	-
ASA_RS02185 (ASA_0413)	pilB	422298..424004 (-)	1707	WP_005314160.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
ASA_RS23765 (ASA_0414)	tapA	424008..424436 (-)	429	WP_005314158.1	type IVa pilus major pilin TapA	-
ASA_RS02195 (ASA_0415)	nadC	424771..425628 (-)	858	WP_005314155.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32500.78 Da
• Isoelectric Point: 6.9383

>NTDB_id=27894 ASA_RS02175 WP_005314162.1 419990..420865(-) (pilD) [Aeromonas salmonicida subsp. salmonicida A449]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEDDERYNLMVPRSCCPRCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide

• Length: 876 bp

>NTDB_id=27894 ASA_RS02175 WP_005314162.1 419990..420865(-) (pilD) [Aeromonas salmonicida subsp. salmonicida A449]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTTTTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCAGAAGATGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCGCTGCAATCAC
CCCATAACGGCCTTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCGCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG
GTACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTGGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A2T195

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	55.556	98.969	0.55
pilD	Acinetobacter baumannii D1279779	55.39	92.44	0.512
pilD	Acinetobacter nosocomialis M2	55.39	92.44	0.512
pilD	Neisseria gonorrhoeae MS11	50.746	92.096	0.467