Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SPYM_RS02535 Genome accession   NC_009332
Coordinates   501262..502587 (+) Length   441 a.a.
NCBI ID   WP_011184811.1    Uniprot ID   -
Organism   Streptococcus pyogenes str. Manfredo     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496262..507587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM_RS02510 (SpyM50459) - 497032..497673 (+) 642 WP_011888671.1 response regulator transcription factor -
  SPYM_RS02515 (SpyM50460) - 497710..499110 (+) 1401 WP_011888672.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SPYM_RS02520 (SpyM50461) - 499110..499487 (+) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  SPYM_RS02525 (SpyM50462) cysK 499505..500446 (-) 942 WP_011888673.1 cysteine synthase A -
  SPYM_RS02530 (SpyM50463) - 500574..501206 (-) 633 WP_009880468.1 YigZ family protein -
  SPYM_RS02535 (SpyM50464) comFA/cflA 501262..502587 (+) 1326 WP_011184811.1 DEAD/DEAH box helicase Machinery gene
  SPYM_RS02540 (SpyM50465) - 502559..503224 (+) 666 WP_002983685.1 ComF family protein -
  SPYM_RS02545 (SpyM50466) hpf 503304..503852 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49788.32 Da        Isoelectric Point: 9.9935

>NTDB_id=27855 SPYM_RS02535 WP_011184811.1 501262..502587(+) (comFA/cflA) [Streptococcus pyogenes str. Manfredo]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEIISQQLLINMQNQKTTLVHAVTGSGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=27855 SPYM_RS02535 WP_011184811.1 501262..502587(+) (comFA/cflA) [Streptococcus pyogenes str. Manfredo]
ATGGAGTCTATTGAAAATAGTTATGGACGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATTGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATAATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTTCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCAAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAAAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(99-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae D39

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae R6

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae TIGR4

53.938

95.011

0.512
  comFA/cflA Streptococcus mitis NCTC 12261

52.607

95.692

0.503
  comFA/cflA Streptococcus mitis SK321

51.659

95.692

0.494
  comFA Lactococcus lactis subsp. cremoris KW2

47.475

89.796

0.426
  comFA Latilactobacillus sakei subsp. sakei 23K

39.08

98.639

0.385

Multiple sequence alignment