Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BURPS668_RS02470 Genome accession   NC_009074
Coordinates   533596..534915 (-) Length   439 a.a.
NCBI ID   WP_004522862.1    Uniprot ID   Q63XM5
Organism   Burkholderia pseudomallei 668     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528596..539915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BURPS668_RS02450 (BURPS668_0557) - 529495..529890 (-) 396 WP_004530943.1 nitrite reductase (NAD(P)H) small subunit -
  BURPS668_RS02455 (BURPS668_0558) nirB 529966..532410 (-) 2445 WP_011851046.1 nitrite reductase large subunit NirB -
  BURPS668_RS30900 (BURPS668_0559) - 532457..532798 (+) 342 WP_079996865.1 hypothetical protein -
  BURPS668_RS02465 (BURPS668_0560) maiA 532795..533439 (+) 645 WP_011851047.1 maleylacetoacetate isomerase -
  BURPS668_RS02470 (BURPS668_0561) pilA 533596..534915 (-) 1320 WP_004522862.1 signal recognition particle-docking protein FtsY Machinery gene
  BURPS668_RS02475 (BURPS668_0563) rsmD 535167..535793 (+) 627 WP_041278203.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BURPS668_RS02480 (BURPS668_0564) coaD 535904..536404 (+) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  BURPS668_RS02485 (BURPS668_0565) - 536467..536733 (+) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BURPS668_RS02490 (BURPS668_0566) hisC 536903..537973 (-) 1071 WP_025986425.1 histidinol-phosphate transaminase -
  BURPS668_RS02495 (BURPS668_0567) pth 538062..538667 (-) 606 WP_004202941.1 aminoacyl-tRNA hydrolase -
  BURPS668_RS02500 (BURPS668_0568) - 538781..539395 (-) 615 WP_011851049.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46312.63 Da        Isoelectric Point: 6.2545

>NTDB_id=27638 BURPS668_RS02470 WP_004522862.1 533596..534915(-) (pilA) [Burkholderia pseudomallei 668]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDERSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLGALREKVRAGRLTDPQQVKDALRGLLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=27638 BURPS668_RS02470 WP_004522862.1 533596..534915(-) (pilA) [Burkholderia pseudomallei 668]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCGCCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCGCA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAACCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCGTCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGACGCGCTGCGCGGGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAAAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCGGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTCCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(240-439)

SRP54-type protein, helical bundle domain (SRP54_N)

(158-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q63XM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.113

100

0.506

Multiple sequence alignment