Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   C5Z25_RS09230 Genome accession   NZ_CP027190
Coordinates   1919924..1921276 (+) Length   450 a.a.
NCBI ID   WP_105452351.1    Uniprot ID   A0A2R3JQQ8
Organism   Lactobacillus sp. CBA3605     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1914924..1926276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5Z25_RS09215 (C5Z25_09215) - 1916786..1917883 (+) 1098 WP_105452348.1 glycosyltransferase family 4 protein -
  C5Z25_RS09220 (C5Z25_09220) - 1918197..1919096 (+) 900 WP_105452349.1 phosphate ABC transporter substrate-binding protein -
  C5Z25_RS09225 (C5Z25_09225) - 1919215..1919856 (-) 642 WP_105452350.1 YigZ family protein -
  C5Z25_RS09230 (C5Z25_09230) comFA 1919924..1921276 (+) 1353 WP_105452351.1 DEAD/DEAH box helicase Machinery gene
  C5Z25_RS09235 (C5Z25_09235) - 1921239..1921913 (+) 675 WP_105452352.1 ComF family protein -
  C5Z25_RS09240 (C5Z25_09240) hpf 1922046..1922612 (+) 567 WP_105452353.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  C5Z25_RS09245 (C5Z25_09245) secA 1922807..1925170 (+) 2364 WP_105452354.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 50847.07 Da        Isoelectric Point: 10.5038

>NTDB_id=275657 C5Z25_RS09230 WP_105452351.1 1919924..1921276(+) (comFA) [Lactobacillus sp. CBA3605]
MQVNELYGRQLNLTVAQASDLPVNTQRFASMQVTTSLVQCQRCHSRLKRAWAQLPNQQYYCYQCLNLGRISTLTSLYHIP
EPNDFPKRPKLTWTGTLTPQQTACAATIQDKFATHQHHLLWAVTGAGKTEMLFPGLAWALAQGLRVAVASPRVDVCLELY
PRLQAAFAGLDIALLHGRQTEPYRYCQLTVCTTHQLLRFRAAFDVLIIDEVDAFPFAANPRLTYAVTQALKTTGALLYLT
ATPSRALLCQVRQHQLSISYLPLRFHQHLLPVIKVTLRPNWWRQIKHGQLPTLLRHWLQEYAHTQRRFLLFVPRVSQLTL
VQAAVQQNIPELTGLTVHSTDPDRLAKVQKMRSATVQYLITTTILERGVTFPGIDVIVLGADDTIFSTAALVQIAGRVGR
SKERPTGLVRFICSSYARPVKQAQRQIKQVNRKGRRLLNELSTVSTTVTT

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=275657 C5Z25_RS09230 WP_105452351.1 1919924..1921276(+) (comFA) [Lactobacillus sp. CBA3605]
ATGCAAGTGAATGAGTTATATGGTCGCCAGCTTAATCTAACAGTCGCTCAGGCTAGCGACTTACCAGTTAATACGCAACG
TTTTGCGAGTATGCAAGTGACCACTAGTTTAGTGCAATGTCAACGTTGTCATAGTCGGTTAAAGCGTGCATGGGCACAAT
TACCCAATCAACAGTATTACTGCTATCAGTGTTTGAATCTGGGCCGGATTAGCACCCTAACTTCACTGTATCATATTCCG
GAACCTAATGACTTTCCTAAGCGGCCTAAATTAACTTGGACTGGTACGTTAACCCCACAACAAACCGCTTGTGCAGCAAC
TATTCAGGATAAGTTTGCGACACATCAACACCACTTACTTTGGGCAGTTACGGGCGCCGGCAAGACCGAGATGTTGTTTC
CGGGGTTAGCTTGGGCATTGGCACAAGGCTTGCGAGTTGCAGTGGCTTCACCACGGGTTGATGTTTGCTTAGAACTATAC
CCGCGGTTACAGGCAGCTTTTGCTGGCTTGGATATTGCCTTACTACATGGGCGACAAACCGAGCCTTATCGTTATTGTCA
ACTGACCGTTTGTACTACACATCAATTGTTGCGATTTCGAGCGGCTTTCGATGTGTTAATTATTGATGAAGTAGATGCGT
TTCCATTTGCTGCTAATCCACGTTTGACTTATGCCGTGACGCAAGCCTTAAAAACAACGGGGGCATTATTATATTTGACA
GCCACGCCTAGTCGAGCGTTGCTGTGCCAAGTACGACAGCATCAGTTAAGTATCAGCTATTTGCCATTACGATTTCATCA
GCATTTATTACCGGTCATTAAGGTAACCCTACGGCCCAATTGGTGGCGTCAAATTAAACACGGCCAGTTACCAACCTTGT
TACGACACTGGTTACAGGAATATGCGCATACGCAGCGTCGATTTTTATTGTTTGTGCCCCGAGTCAGTCAACTGACATTG
GTTCAGGCGGCCGTGCAACAAAATATTCCGGAATTAACCGGTTTAACAGTGCATTCAACTGATCCTGACCGGTTGGCGAA
AGTTCAGAAGATGCGTTCGGCGACAGTACAATATTTAATTACGACGACGATTTTAGAACGTGGCGTGACGTTTCCTGGTA
TTGATGTGATTGTGTTAGGTGCTGATGATACCATTTTTTCAACGGCAGCGTTAGTTCAGATTGCAGGGCGCGTAGGTCGT
TCAAAAGAGCGGCCCACTGGCTTAGTGCGTTTTATTTGTAGCAGTTACGCACGGCCAGTCAAACAAGCCCAACGCCAAAT
TAAGCAAGTTAATCGGAAAGGTCGGCGCTTACTAAATGAACTGTCTACTGTGTCAACGACCGTTACAACCTGA

Domains

Predicted by InterProScan.

Type III restriction enzyme, res subunit (ResIII)

(99-245)

Helicase conserved C-terminal domain (Helicase_C)

(300-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

49.543

97.333

0.482
  comFA/cflA Streptococcus mitis NCTC 12261

40.678

91.778

0.373
  comFA Bacillus subtilis subsp. subtilis str. 168

40.64

90.222

0.367
  comFA/cflA Streptococcus pneumoniae TIGR4

39.609

90.889

0.36
  comFA/cflA Streptococcus pneumoniae D39

39.609

90.889

0.36
  comFA/cflA Streptococcus pneumoniae Rx1

39.609

90.889

0.36
  comFA/cflA Streptococcus pneumoniae R6

39.609

90.889

0.36
  comFA Lactococcus lactis subsp. cremoris KW2

39.706

90.667

0.36

Multiple sequence alignment