Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   C5695_RS12630 Genome accession   NZ_CP027116
Coordinates   2426313..2427083 (-) Length   256 a.a.
NCBI ID   WP_117731032.1    Uniprot ID   A0AAD0HNS9
Organism   Bacillus pumilus strain 145     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2421313..2432083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5695_RS12610 (C5695_12660) - 2421407..2422228 (-) 822 WP_117731030.1 pyruvate, water dikinase regulatory protein -
  C5695_RS12615 (C5695_12665) - 2422253..2422891 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  C5695_RS12620 (C5695_12670) glyS 2423058..2425103 (-) 2046 WP_117731031.1 glycine--tRNA ligase subunit beta -
  C5695_RS12625 (C5695_12675) glyQ 2425096..2425983 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  C5695_RS12630 (C5695_12680) recO 2426313..2427083 (-) 771 WP_117731032.1 DNA repair protein RecO Machinery gene
  C5695_RS12635 (C5695_12685) - 2427119..2427262 (-) 144 WP_003217288.1 YqzL family protein -
  C5695_RS12640 (C5695_12690) era 2427411..2428316 (-) 906 WP_007501305.1 GTPase Era -
  C5695_RS12645 (C5695_12695) - 2428309..2428707 (-) 399 WP_034661347.1 cytidine deaminase -
  C5695_RS12650 (C5695_12700) - 2428821..2429207 (-) 387 WP_117731033.1 diacylglycerol kinase family protein -
  C5695_RS12655 (C5695_12705) ybeY 2429185..2429664 (-) 480 WP_117731034.1 rRNA maturation RNase YbeY -
  C5695_RS12660 (C5695_12710) - 2429665..2431815 (-) 2151 WP_117731035.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29171.87 Da        Isoelectric Point: 8.4348

>NTDB_id=275062 C5695_RS12630 WP_117731032.1 2426313..2427083(-) (recO) [Bacillus pumilus strain 145]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELLDKGIEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGSVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=275062 C5695_RS12630 WP_117731032.1 2426313..2427083(-) (recO) [Bacillus pumilus strain 145]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCTAAAAAATCAAATAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGGCTTGGAACGCTCCAGCAAGGCGAAATGATCGAAAGTATGCGGGCC
ATTCGTGAGGATTTATTTCTAACCGCATATGCTGCATATATGACAGAGTTACTCGATAAAGGCATAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAGCTCCTGCTTCAATCTCTGCGCCATCTCAATGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTAATGGGGATGAAGCCTGAACTGGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATTAGAGATAATGGATTCATTTGCCAAAATTGCTTTTCTAAAGATCCTTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTCGGTTCGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTATTATGACGAATATTCAGGCCTTTATTTAAAATCAAAAAAATTCATG
AATCAAATGGAATCCATGAAAAAGCTTATGGGCGGCGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75

100

0.75

Multiple sequence alignment