Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C5695_RS00590 Genome accession   NZ_CP027116
Coordinates   109220..110599 (+) Length   459 a.a.
NCBI ID   WP_117728239.1    Uniprot ID   -
Organism   Bacillus pumilus strain 145     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104220..115599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5695_RS00570 (C5695_00570) - 104562..105026 (+) 465 WP_034323278.1 CtsR family transcriptional regulator -
  C5695_RS00575 (C5695_00575) - 105041..105598 (+) 558 WP_117728233.1 UvrB/UvrC motif-containing protein -
  C5695_RS00580 (C5695_00580) - 105603..106694 (+) 1092 WP_117728235.1 protein arginine kinase -
  C5695_RS00585 (C5695_00585) clpC 106691..109126 (+) 2436 WP_117728237.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  C5695_RS00590 (C5695_00590) radA 109220..110599 (+) 1380 WP_117728239.1 DNA repair protein RadA Machinery gene
  C5695_RS00595 (C5695_00595) disA 110602..111681 (+) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  C5695_RS00600 (C5695_00600) - 111835..112935 (+) 1101 WP_117732955.1 PIN/TRAM domain-containing protein -
  C5695_RS00605 (C5695_00605) ispD 112949..113638 (+) 690 WP_117728241.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C5695_RS00610 (C5695_00610) ispF 113642..114118 (+) 477 WP_117728243.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49995.46 Da        Isoelectric Point: 7.3829

>NTDB_id=275030 C5695_RS00590 WP_117728239.1 109220..110599(+) (radA) [Bacillus pumilus strain 145]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLSEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=275030 C5695_RS00590 WP_117728239.1 109220..110599(+) (radA) [Bacillus pumilus strain 145]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTCCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTCC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCAGAAGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGGAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTATTATACATATCTGGTGAGGAGTCCATTAAACAAA
CAAAGCTAAGAGCGGACCGCCTCGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTTGTGGTGGATTCGATTCAAACTGTTTATCAAAGCGATATTACATCCGC
TCCTGGATCTGTCTCTCAAGTCAGAGAATGCACAGCACAGCTGATGAAAATCGCCAAGACAAATGGGATTCCTATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGCCCGCGTCTTTTAGAGCACATGGTGGACACGGTGCTTTATTTT
GAAGGTGAGCGTCATCATACGTTTCGTATCTTGCGTGCGGTGAAAAACCGATTTGGTTCAACGAATGAATTAGGTATTTT
TGAAATGAGAGAGGAAGGGCTCTCAGAAGTATTAAACCCATCAGAAATTTTCTTAGAAGAGCGGTCAGCAGGTGTTTCTG
GATCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTTGTCGAGATACAGGCATTGATTTCCCCGACAAGCTTC
GGGAATCCGCGAAGAATGGCCACTGGCCTCGATCATAATCGAGTATCGCTGCTCATGGCAGTTTTAGAAAAACGTGTCGG
ATTGCTTTTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTGGCTA
TTGCCGTCAGTATTGCTTCAAGCTTTAAAGACGCAGCGCCGCATCAAGCAGATTGCTTTATAGGAGAGGTTGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCAAAACTAGGATTTAAGAGAATGTTTATTCC
TCAGGCGAATATAGATGGTTGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment