Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   C4M74_RS09925 Genome accession   NZ_CP026744
Coordinates   2063296..2063841 (-) Length   181 a.a.
NCBI ID   WP_104227653.1    Uniprot ID   -
Organism   Pasteurella multocida strain 161215033201-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2058296..2068841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4M74_RS09905 - 2059159..2059770 (-) 612 WP_083004649.1 YfgM family protein -
  C4M74_RS09910 hisS 2059789..2061060 (-) 1272 WP_104227651.1 histidine--tRNA ligase -
  C4M74_RS09915 ispG 2061090..2062193 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  C4M74_RS09920 - 2062220..2063173 (-) 954 WP_083004644.1 RodZ family helix-turn-helix domain-containing protein -
  C4M74_RS09925 pilF/pilF2 2063296..2063841 (-) 546 WP_104227653.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C4M74_RS09930 - 2063881..2065065 (-) 1185 WP_083004640.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  C4M74_RS09935 - 2065245..2066498 (-) 1254 WP_005725114.1 AI-2E family transporter -
  C4M74_RS09940 glnB 2066499..2066837 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  C4M74_RS09945 mog 2066882..2067460 (-) 579 WP_005725110.1 molybdopterin adenylyltransferase -
  C4M74_RS09950 - 2067487..2068416 (-) 930 WP_059245741.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20847.43 Da        Isoelectric Point: 7.9056

>NTDB_id=272128 C4M74_RS09925 WP_104227653.1 2063296..2063841(-) (pilF/pilF2) [Pasteurella multocida strain 161215033201-1]
MTRVFQLGIASIFSLFLSACVSQEGHKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFVKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=272128 C4M74_RS09925 WP_104227653.1 2063296..2063841(-) (pilF/pilF2) [Pasteurella multocida strain 161215033201-1]
ATGACGCGCGTTTTTCAATTAGGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTCACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGTCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACACTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459

Multiple sequence alignment