Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NLP_RS28440 Genome accession   NZ_CP026692
Coordinates   6410371..6412377 (+) Length   668 a.a.
NCBI ID   WP_104909245.1    Uniprot ID   -
Organism   Nostoc sp. 'Lobaria pulmonaria (5183) cyanobiont' strain 5183     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6405371..6417377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLP_RS28430 (NLP_5493) dnaK 6407068..6409026 (-) 1959 WP_104909243.1 molecular chaperone DnaK -
  NLP_RS28435 (NLP_5494) grpE 6409255..6410001 (-) 747 WP_104909244.1 nucleotide exchange factor GrpE -
  NLP_RS28440 (NLP_5495) pilB/pilB1 6410371..6412377 (+) 2007 WP_104909245.1 GspE/PulE family protein Machinery gene
  NLP_RS28445 (NLP_5496) pilT 6412485..6413612 (+) 1128 WP_104909246.1 type IV pilus twitching motility protein PilT Machinery gene
  NLP_RS28450 (NLP_5497) - 6413692..6414915 (+) 1224 WP_104909247.1 type II secretion system F family protein -
  NLP_RS28455 (NLP_5498) - 6415061..6415621 (+) 561 WP_104909248.1 hypothetical protein -
  NLP_RS28460 (NLP_5499) - 6415994..6417088 (+) 1095 WP_199784892.1 serine/threonine-protein kinase -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74472.37 Da        Isoelectric Point: 5.5386

>NTDB_id=271752 NLP_RS28440 WP_104909245.1 6410371..6412377(+) (pilB/pilB1) [Nostoc sp. 'Lobaria pulmonaria (5183) cyanobiont' strain 5183]
MTYSSAQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSVGNTMMGNLIETLIPVDICRRHRLVPLSKHKDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVRQKHLERENSTDISQDLENLGNLDLADAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLREAFPPMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLDIVKDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRVPYTPTTEELARYGLSASSDVGVTFYKANTLTLDAIAEAKTKNHLCPKCNG
VGYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTARF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=271752 NLP_RS28440 WP_104909245.1 6410371..6412377(+) (pilB/pilB1) [Nostoc sp. 'Lobaria pulmonaria (5183) cyanobiont' strain 5183]
ATGACTTACTCGTCAGCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTCGGCAACAAGCTAGT
GCAATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCAGACCCTTAACAGAAG
TATTAGAGTCAATCACTGGTCAACAACTATCACCTGAGCTGCTCAGGCAATATAAAAAACAGCAACTATTTGAACTTAAA
ATACTATACGGAGTTGAATTCCTCGATCCAGAAGTCAATTCCGTTGGCAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTGGATATCTGCCGTCGCCATCGCTTAGTACCACTGTCGAAACACAAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTTGCTCCAGATAATCTAGAGGCTTCCGATGACCTGAACCGCATCTTGCGCCCCCAAGGCTTGGCGTTGCAG
CGCATGGTGATTACACAGGAAGACTACCAGCAGCTAATCAACCAATACCTGGATGAAATGGCTGTTCGGCAAAAACACCT
AGAACGAGAAAACTCTACAGATATTAGTCAAGATTTAGAAAATCTTGGAAATCTCGATCTTGCGGATGCTCCTGAAGAAA
TGGAGGCTGATCTAGGGGCAGCGATGAAGGGCGCAGAGGATGCCCCAGTCATCAACCTAGTTAATAGAATCCTAGCTAAA
GCTTTGCATGAGGGCGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGCTTTCGGAAAGATGGGGT
ACTGCGTGAAGCTTTCCCCCCAATGCCGAAAAAAATCATCCCGGCGGTGACAGCCCGATTTAAAATCATCTCCAATCTAG
ACATTGCTGAACGGCGTCTACCCCAAGATGGACGTATCCGGCGGCTGTTTGAGGGACGTAAGGTGGACTTCCGGGTAAAT
ACCTTACCCAGTCGCTACGGGGAAAAGGTGGTGCTGCGGATTTTGGATAACTCCTCCACCCAGTTGGGATTGAATAAGTT
AATTACCGATCCAGAGACTTTGGATATTGTCAAGGATATGGTCAGCCGTCCCTTTGGGCTAATTTTGGTAACTGGGCCAA
CTGGTTCTGGGAAAACAACTTCGCTGTATTCTGCACTCTCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTGGAA
GACCCAATTGAGTATAGTCTTCCAGGCATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTAGATTTTGCTACAGCTCT
GCGGGCTTTCTTACGGCAAGATCCAGATGTGCTGCTAGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCAATTG
AGGCTGCCTTGACCGGTCACTTAGTATTAACTACCTTACATACCAATGATGCCCCAGGAGCGATCGCTCGTTTGGGAGAA
ATGGGGATTGAGCCTTTCATGGTTTCTAGTTCCCTAATTGGTGTTTTAGCTCAACGTTTGGTGCGGCGTGTATGTTCTGA
ATGTCGTGTTCCCTACACTCCCACAACTGAGGAACTGGCTCGTTATGGTCTATCAGCTTCTTCAGATGTGGGAGTCACCT
TCTACAAAGCTAACACCTTGACATTAGATGCGATCGCCGAAGCCAAAACTAAAAATCATCTTTGCCCAAAATGTAATGGT
GTCGGCTACAAAGGGCGTTGTGGTGTTTATGAAGTCATGCGAGTCACCGAAAACCTGCAAACTCTGATCAACGAAGATGC
ACCCACGGAACGCATCAAGGAAGTGGCAATAGAAGAGGGGATGAAAACCCTACTGGCTTATAGTTTGGACTTAGTGCGTG
AAGGTTCAACCACTTTAGAAGAAGTAGAACGAGTGACGTTTACTGATACTGGCTTGGAAGCAGAGTTAAAAGCCAAACGC
AAGACAGGTCTTACCTGCCGGAGTTGCGATGCCACATTGAAACAAGAATGGTTGGATTGTCCTTACTGTATGACAGCTCG
GTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.327

100

0.657
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.737

91.168

0.362

Multiple sequence alignment