Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   C2I25_RS18110 Genome accession   NZ_CP026678
Coordinates   3415400..3416443 (+) Length   347 a.a.
NCBI ID   WP_106824083.1    Uniprot ID   -
Organism   Bacillus cereus strain TG1-6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3410400..3421443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2I25_RS18075 (C2I25_18055) - 3410765..3411253 (+) 489 WP_145959752.1 hypothetical protein -
  C2I25_RS18080 (C2I25_18060) - 3411288..3411599 (-) 312 WP_001093240.1 hypothetical protein -
  C2I25_RS18085 (C2I25_18065) - 3411853..3412068 (+) 216 WP_001008326.1 DUF3912 family protein -
  C2I25_RS29155 - 3412156..3412548 (+) 393 Protein_3356 hypothetical protein -
  C2I25_RS18095 (C2I25_18075) - 3412628..3414022 (-) 1395 WP_001094344.1 L-cystine transporter -
  C2I25_RS18100 (C2I25_18080) - 3414130..3414372 (-) 243 WP_000375619.1 DUF2626 domain-containing protein -
  C2I25_RS18105 (C2I25_18085) - 3414499..3415194 (-) 696 WP_000434107.1 metalloregulator ArsR/SmtB family transcription factor -
  C2I25_RS18110 (C2I25_18090) comGA 3415400..3416443 (+) 1044 WP_106824083.1 competence type IV pilus ATPase ComGA Machinery gene
  C2I25_RS18115 (C2I25_18095) comGB 3416430..3417467 (+) 1038 WP_106825182.1 competence type IV pilus assembly protein ComGB -
  C2I25_RS18120 (C2I25_18100) comGC 3417479..3417778 (+) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  C2I25_RS18125 (C2I25_18105) comGD 3417775..3418230 (+) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  C2I25_RS18130 (C2I25_18110) - 3418223..3418525 (+) 303 WP_000829467.1 hypothetical protein -
  C2I25_RS18135 (C2I25_18115) comGF 3418495..3418965 (+) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  C2I25_RS18140 (C2I25_18120) comGG 3418962..3419333 (+) 372 WP_106824087.1 competence type IV pilus minor pilin ComGG -
  C2I25_RS18145 (C2I25_18125) - 3419507..3420157 (+) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  C2I25_RS18150 (C2I25_18130) aroK 3420273..3420770 (+) 498 WP_106824089.1 shikimate kinase AroK -
  C2I25_RS18155 (C2I25_18135) - 3420809..3421009 (+) 201 WP_000106083.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39105.49 Da        Isoelectric Point: 8.3562

>NTDB_id=271674 C2I25_RS18110 WP_106824083.1 3415400..3416443(+) (comGA) [Bacillus cereus strain TG1-6]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTGHCIEKEFGEKLVLHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=271674 C2I25_RS18110 WP_106824083.1 3415400..3416443(+) (comGA) [Bacillus cereus strain TG1-6]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGGGACATTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTTACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGCCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCGAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGGGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGCAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATAAGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

39.441

92.795

0.366

Multiple sequence alignment