Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   D0808_RS10005 Genome accession   NZ_CP031783
Coordinates   1923998..1926097 (-) Length   699 a.a.
NCBI ID   WP_153255975.1    Uniprot ID   -
Organism   Bacillus subtilis strain MENO2 voucher National Research Centre     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1918998..1931097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0808_RS09975 (D0808_10065) spo0E 1919054..1919311 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  D0808_RS09980 (D0808_10070) - 1919397..1919828 (+) 432 WP_003245685.1 hypothetical protein -
  D0808_RS09985 (D0808_10075) kinD 1919856..1921376 (-) 1521 WP_072557174.1 sporulation kinase KinD -
  D0808_RS09990 (D0808_10080) mhqR 1921569..1922006 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  D0808_RS09995 (D0808_10085) motB 1922046..1922831 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  D0808_RS10000 (D0808_10090) motA 1922803..1923615 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  D0808_RS10005 (D0808_10095) clpE 1923998..1926097 (-) 2100 WP_153255975.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  D0808_RS10010 (D0808_10100) - 1926463..1927506 (+) 1044 WP_076457776.1 hypothetical protein -
  D0808_RS10015 (D0808_10105) queC 1927820..1928479 (+) 660 WP_014476630.1 7-cyano-7-deazaguanine synthase QueC -
  D0808_RS10020 (D0808_10110) queD 1928472..1928921 (+) 450 WP_080009794.1 6-carboxytetrahydropterin synthase QueD -
  D0808_RS10025 (D0808_10115) queE 1928914..1929645 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  D0808_RS10030 (D0808_10120) queF 1929663..1930160 (+) 498 WP_003218613.1 preQ(1) synthase -
  D0808_RS10035 (D0808_10125) ykvN 1930679..1931035 (-) 357 WP_015715792.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77773.98 Da        Isoelectric Point: 5.4428

>NTDB_id=271123 D0808_RS10005 WP_153255975.1 1923998..1926097(-) (clpE) [Bacillus subtilis strain MENO2 voucher National Research Centre]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEKSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
NEDAAERLTAIEAEKTKALEDENYELAAKLRDEELALKKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTA
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=271123 D0808_RS10005 WP_153255975.1 1923998..1926097(-) (clpE) [Bacillus subtilis strain MENO2 voucher National Research Centre]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAAAAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCTCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGTTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTTGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
AATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGATGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGAAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAGCTTGAAGCAAAACTTCATGAACGCGTGATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGTAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATACGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAAAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGACGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACAGTGATCATCATGACAAGCAAC
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGCT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)

AAA lid domain (AAA_lid_9)

(253-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.528

100

0.601
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpE Streptococcus pneumoniae TIGR4

54.646

100

0.564
  clpC Bacillus subtilis subsp. subtilis str. 168

54.028

90.558

0.489
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.544

90.272

0.429
  clpC Streptococcus pneumoniae Rx1

46.562

91.559

0.426
  clpC Streptococcus pneumoniae D39

46.562

91.559

0.426
  clpC Streptococcus thermophilus LMD-9

45.038

93.705

0.422
  clpC Streptococcus pneumoniae TIGR4

47.173

88.555

0.418
  clpC Streptococcus thermophilus LMG 18311

44.58

93.705

0.418
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.914

86.695

0.363

Multiple sequence alignment