Detailed information NTG0270297

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BKC06_RS04375
• Genome accession: NZ_CP026568
• Coordinates: 912915..913787 (-)
• Length: 290 a.a.
• NCBI ID: WP_054092017.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae strain Pss9097
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 907915..918787

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKC06_RS04340 (BKC06_004340)	-	908700..909998 (-)	1299	WP_003430739.1	NAD(P)/FAD-dependent oxidoreductase	-
BKC06_RS04350 (BKC06_004350)	-	910215..910748 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
BKC06_RS04355 (BKC06_004355)	-	910745..911374 (-)	630	WP_003430735.1	DUF1780 domain-containing protein	-
BKC06_RS04360 (BKC06_004360)	-	911501..911962 (+)	462	WP_054092019.1	hypothetical protein	-
BKC06_RS04365 (BKC06_004365)	yacG	912089..912298 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
BKC06_RS04370 (BKC06_004370)	coaE	912295..912918 (-)	624	WP_004415092.1	dephospho-CoA kinase	-
BKC06_RS04375 (BKC06_004375)	pilD	912915..913787 (-)	873	WP_054092017.1	prepilin peptidase	Machinery gene
BKC06_RS04380 (BKC06_004380)	pilC	913789..915006 (-)	1218	WP_032654583.1	type II secretion system F family protein	Machinery gene
BKC06_RS04385 (BKC06_004385)	pilB	915009..916703 (-)	1695	WP_003406565.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BKC06_RS04390 (BKC06_004390)	-	916927..917355 (+)	429	WP_003406568.1	pilin	-
BKC06_RS27950	-	917656..917763 (-)	108	Protein_810	transcriptional regulator	-
BKC06_RS27625 (BKC06_004395)	-	917929..918345 (+)	417	WP_233902709.1	glutathione binding-like protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31969.12 Da
• Isoelectric Point: 8.3734

>NTDB_id=270297 BKC06_RS04375 WP_054092017.1 912915..913787(-) (pilD) [Pseudomonas syringae pv. syringae strain Pss9097]
MSLLDLLASSPLAFVTTCCILGLIIGSFLNVIVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=270297 BKC06_RS04375 WP_054092017.1 912915..913787(-) (pilD) [Pseudomonas syringae pv. syringae strain Pss9097]
ATGTCCCTCCTCGACCTCCTGGCCAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGATCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGAGAACTGCTCGGCC
TGCCTGCTGAACCCGATCAACCCGTCTTCAATCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCTTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGTAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTATGTGGCTTGGCATTTCGGTTTTGGCTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTTACCTCGCTGAACGATGCGCTGTGGGGAGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGGTTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGTGTCCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.19	94.483	0.493
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472