Detailed information NTG0270238

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BKM19_RS06490
• Genome accession: NZ_CP026558
• Coordinates: 1193812..1194684 (-)
• Length: 290 a.a.
• NCBI ID: WP_060404277.1
• Uniprot ID: A0AB35R099
• Organism: Pseudomonas amygdali pv. morsprunorum strain R15244
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1190871..1199739	1193812..1194684	within	0

Gene organization within MGE regions

Location: 1190871..1199739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKM19_RS06475 (BKM19_006465)	-	1192390..1192836 (+)	447	WP_099456531.1	hypothetical protein	-
BKM19_RS06480 (BKM19_006470)	yacG	1192986..1193195 (-)	210	WP_060404278.1	DNA gyrase inhibitor YacG	-
BKM19_RS06485 (BKM19_006475)	coaE	1193192..1193815 (-)	624	WP_057422075.1	dephospho-CoA kinase	-
BKM19_RS06490 (BKM19_006480)	pilD	1193812..1194684 (-)	873	WP_060404277.1	prepilin peptidase	Machinery gene
BKM19_RS06495 (BKM19_006485)	pilC	1194686..1195903 (-)	1218	WP_054995403.1	type II secretion system F family protein	Machinery gene
BKM19_RS06500 (BKM19_006490)	pilB	1195906..1197600 (-)	1695	WP_060404276.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BKM19_RS06505 (BKM19_006495)	-	1197824..1198252 (+)	429	WP_060404275.1	pilin	-
BKM19_RS06510 (BKM19_006500)	-	1198548..1198883 (-)	336	Protein_1110	DUF1523 family protein	-
BKM19_RS06515 (BKM19_006505)	-	1199152..1199739 (+)	588	WP_161938281.1	DUF433 domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32057.19 Da
• Isoelectric Point: 8.5797

>NTDB_id=270238 BKM19_RS06490 WP_060404277.1 1193812..1194684(-) (pilD) [Pseudomonas amygdali pv. morsprunorum strain R15244]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPKMMERDWKAQSRELLGLPAEPDQPAFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIINAFGLFNSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPFTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=270238 BKM19_RS06490 WP_060404277.1 1193812..1194684(-) (pilD) [Pseudomonas amygdali pv. morsprunorum strain R15244]
ATGCCCCTCCTCGACCTACTGGCCAGCTCCCCTCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTCATCATCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTTCCCAAAATGATGGAGCGCGACTGGAAAGCCCAATCCCGAGAACTGCTCGGCC
TGCCCGCAGAACCCGACCAGCCTGCCTTCAACCTGAACCGCCCTCGCTCAAGCTGCCCGCACTGCGCGCACAAGATCCGC
CCGTGGGAGAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCACAATGCAAAGCCCCCATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGCTTCGGCTGGCAGGCAGCGG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGGCGATGAGTCTGATCGACGCCGACCACCAGCTGTTACCTGACTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCATCAACGCCTTCGGGCTATTCAACTCGCTGAACGATGCGCTGTGGGGTGCAGT
GGCCGGGTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTTACCATCCTGCTGTCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.521	97.931	0.524
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476