Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DOM02_RS11375 Genome accession   NZ_CP031677
Coordinates   2427226..2428953 (-) Length   575 a.a.
NCBI ID   WP_019726393.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain E80     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2422226..2433953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOM02_RS11345 (DOM02_11280) - 2423086..2424165 (-) 1080 WP_003119955.1 type II secretion system protein GspK -
  DOM02_RS11350 (DOM02_11285) - 2424155..2424745 (-) 591 WP_003097720.1 type II secretion system protein -
  DOM02_RS11355 (DOM02_11290) - 2424742..2425167 (-) 426 WP_019726391.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DOM02_RS11360 (DOM02_11295) - 2425167..2425577 (-) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DOM02_RS11365 (DOM02_11300) gspG 2425546..2425980 (-) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  DOM02_RS11370 (DOM02_11305) - 2426034..2427221 (-) 1188 WP_023081364.1 type II secretion system F family protein -
  DOM02_RS11375 (DOM02_11310) pilF 2427226..2428953 (-) 1728 WP_019726393.1 GspE/PulE family protein Machinery gene
  DOM02_RS11380 (DOM02_11315) - 2428943..2429746 (-) 804 WP_019726394.1 ABC transporter permease -
  DOM02_RS11385 (DOM02_11320) - 2430338..2431090 (-) 753 WP_003110095.1 class I SAM-dependent methyltransferase -
  DOM02_RS11390 (DOM02_11325) - 2431316..2432290 (+) 975 WP_019726395.1 quinone oxidoreductase -
  DOM02_RS11395 (DOM02_11330) - 2432313..2433242 (+) 930 WP_003160458.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63696.75 Da        Isoelectric Point: 5.3299

>NTDB_id=269449 DOM02_RS11375 WP_019726393.1 2427226..2428953(-) (pilF) [Pseudomonas aeruginosa strain E80]
MSSEAVSPGPQTTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TQAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=269449 DOM02_RS11375 WP_019726393.1 2427226..2428953(-) (pilF) [Pseudomonas aeruginosa strain E80]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGACGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCACTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGTTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGTTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGTTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCCAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGGG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.519

98.435

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365