Detailed information NTG0268840

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BV11031_RS12805
• Genome accession: NZ_CP026362
• Coordinates: 2369680..2373384 (-)
• Length: 1234 a.a.
• NCBI ID: WP_010329299.1
• Uniprot ID: -
• Organism: Bacillus vallismortis strain DSM 11031
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2364680..2378384

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BV11031_RS12790 (BV11031_12770)	-	2364726..2365028 (-)	303	WP_129550771.1	HNH endonuclease	-
BV11031_RS12795 (BV11031_12775)	sbcC	2365042..2368434 (-)	3393	WP_129550772.1	exonuclease subunit SbcC	-
BV11031_RS12800 (BV11031_12780)	sbcD	2368431..2369606 (-)	1176	WP_010329300.1	exonuclease subunit SbcD	-
BV11031_RS12805 (BV11031_12785)	addA	2369680..2373384 (-)	3705	WP_010329299.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BV11031_RS12810 (BV11031_12790)	addB	2373371..2376871 (-)	3501	WP_129550773.1	helicase-exonuclease AddAB subunit AddB	Machinery gene

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141605.86 Da
• Isoelectric Point: 5.0227

>NTDB_id=268840 BV11031_RS12805 WP_010329299.1 2369680..2373384(-) (addA) [Bacillus vallismortis strain DSM 11031]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVNRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYIKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFNELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLIRLVTDFGKRFEAAKQEKSIIDFSDLEHYCLSILTAEDDKGEQKPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RTDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEETDANEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMDELRAQGIPVYANLTSGYYDAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYTHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYIGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRITYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQVAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHKDVEDLAENVMAAPADISSHPARFAVRMIHSYD
LLDDDLEERMEEKSERLEAIRRGEPVPGSFTFDEKAREQLSWTYPHHEVTHIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTVEEAEQTVHKLFEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLIDAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCFYETENGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIANTNVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=268840 BV11031_RS12805 WP_010329299.1 2369680..2373384(-) (addA) [Bacillus vallismortis strain DSM 11031]
ATGAACATTCCTAAACCGGCAGACAGCACTTGGACAGATGACCAATGGAATGCCATTGTTTCAACGGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGAAAAACCGCTGTGCTCGTCGAACGAATGATCCGGAAGATCACCGCGGAGGAAAACC
CGATTGATGTGGACCGGCTTCTCGTTGTGACGTTTACAAATGCCTCAGCGGCAGAGATGAAACACCGGATTGCGGATGCC
TTGGAAAAAGAGCTTGTCAACCGGCCAGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACAGGGCCAGCATTTC
GACACTGCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAAACAGAAGGCGAACTTTTGGGGGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAAGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCATGATCTGGACCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCAAATCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATATCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTCCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGTCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTCGCGCAGATTGATGAACT
GATTCAGCACCAGGATGATTTCAATGAGCTTTATAAGCGGGTGCCGGCAGTCTCCTTTAAGCGCGCGAAAGCGGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACGAGAAGTCCTGAACAGCATTTGAAAAGCTTAGCCGAGATGAAGCCTGTCATTGAAACGCTCATACG
GCTTGTCACCGACTTTGGAAAACGGTTCGAAGCCGCGAAACAGGAAAAATCAATCATTGATTTTTCTGATTTAGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGGATGATAAAGGTGAACAGAAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAAGATACCAACTTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
CGAGGAAACAGGCAACCTATTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTGGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAAAGCGGAGAAGGCACGGGCCGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGGACTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGATGA
GCAGGCTGAACTGAAGCTTGGCGCGGCGTATCCGGACAATGATGAGACGGAAACGGAGCTGCTGCTGATTGACAACGCCG
AAGAGACGGATGCAAACGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTTGAAGCGAAAGCGATTGCCAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAAGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAGTACCGCGATATCGTGAT
TTTGCTTCGTTCCATGCCGTGGGCGCCGCAAATCATGGACGAGCTGAGGGCGCAGGGTATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTATGATGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAATCCGTATCAGGATATA
CCGCTGGCCTCTGTGTTGCGCTCACCTATTGTCGGGGCGGATGAAAACGAGCTGTCTTTGATCAGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGATTACTTGGCGGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACAT
TCTACACTCACCTGCAAAAATGGCGCGCCTTTTCAAAAAACCACTCCGTATCCGAACTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATATCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCCCGCGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTATATTCATCCGCAACTGCGCATCACCTATCCAACGCTGCCACTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTTGCCTTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCATGCAAGGATCATCAGAAACAGGTCGCAAAATGGCAGGCATCTGCCTCCCAAACGGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATCGGTCCGGCTCTTGCCAGACACAAAGATGTAGAGGATTTGG
CTGAAAACGTTATGGCAGCGCCTGCCGACATATCAAGCCACCCGGCTCGTTTTGCCGTTCGTATGATCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGTTTAGAAGCGATACGCCGTGGTGAACCAGTTCC
TGGCTCGTTTACGTTTGATGAAAAAGCCCGTGAGCAGCTGAGCTGGACATATCCTCATCACGAGGTGACGCACATTCGGA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGTTAAACCGGCT
GACGGAAGCATGCTGTACAGGCGGCCCGCTTTCATGATGAAAAAAGGCCTGACAGCAGCCGAAAAAGGGACGGCCATGCA
TACAGTGATGCAGCATATCCCGCTGACACATGTGCCGACAGTAGAGGAAGCGGAGCAAACGGTTCATAAGCTCTTTGAAA
AGGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGACGCTAAATGGAAGGACCGGGAAGTGCCGTTTAGTTTGGCGCTTCCGGCCAAGGAAATCTACCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTGGTGCAGGGAATTATCGACTGTTTCTATGAAACTGAAAACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGGGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAACACAAACGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGTCACATTCTGACATTATAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-882)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	95.219	100	0.952