Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   C0971_RS04485 Genome accession   NZ_CP026143
Coordinates   879758..883540 (+) Length   1260 a.a.
NCBI ID   WP_248893055.1    Uniprot ID   -
Organism   Bacillus methanolicus strain DFS2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 874758..888540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS04470 (C0971_04530) - 874787..875398 (+) 612 WP_248893048.1 TVP38/TMEM64 family protein -
  C0971_RS04475 (C0971_04535) lepB 875436..875990 (+) 555 WP_248893050.1 signal peptidase I -
  C0971_RS04480 (C0971_04540) addB 876274..879798 (+) 3525 WP_248893052.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  C0971_RS04485 (C0971_04545) addA 879758..883540 (+) 3783 WP_248893055.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  C0971_RS04490 (C0971_04550) - 883583..883804 (-) 222 WP_004433484.1 spore germination protein -
  C0971_RS04495 (C0971_04555) - 883870..884259 (-) 390 WP_248893057.1 spore germination protein GerPE -
  C0971_RS04500 (C0971_04560) - 884261..884443 (-) 183 WP_004433491.1 hypothetical protein -
  C0971_RS04505 (C0971_04565) gerPC 884440..885048 (-) 609 WP_004433494.1 spore germination protein GerPC -
  C0971_RS04510 (C0971_04570) - 885071..885289 (-) 219 WP_248893059.1 spore germination protein GerPB -
  C0971_RS04515 (C0971_04575) - 885307..885528 (-) 222 WP_004433500.1 spore germination protein -
  C0971_RS04520 (C0971_04580) - 885981..887171 (+) 1191 WP_248893061.1 DUF418 domain-containing protein -
  C0971_RS04525 (C0971_04585) - 887213..888124 (+) 912 WP_248893063.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1260 a.a.        Molecular weight: 145305.74 Da        Isoelectric Point: 4.9186

>NTDB_id=267504 C0971_RS04485 WP_248893055.1 879758..883540(+) (addA) [Bacillus methanolicus strain DFS2]
MILTNSVIIPPKPENVTWTDDQWKAIMAKDRDILVAAAAGSGKTAVLVERIIRRITSLHDPIDVDQLLVVTFTNASAAEM
RHRIGEALEKEINQNPTSAHLRKQLSLLNKASISTLHSFCLEVIKKYYYLIDIDPGFRIADETEAQLMRDEVLDELFEEE
YGKSDNEAFFSLVDTFTNDRSDEALQDIIRELYDFARSNPSPDRYLKSIIEMYDVDENGKLEDLPFMDALLDDIEMQLEG
AKQMLLEGLELTKLPGGPAPRAENFMDDLHIVDTLISAKNDSWSTLYNAMQNLSFSRAKTVRGDEYDGDLVEKAQKLRER
AKKVLQDLQSELFSRRPESFLKDMREMKGHIETLISLVKSFSDRFEQVKREKGLVDFADLEHYCLDILTGSIDEDGRRIP
SEAALSYRRLFKEVLVDEYQDTNMVQEAILQLVTNEEEQTGNLFMVGDVKQSIYRFRLAEPNLFLSKYNRFKPTGADSGL
RIDLARNFRSRKEVLDGTNFLFKQFMGVKVGEIDYDEAAELKKGAPYPEETSNPVSVILIDQAGEDDESDADELPEADNI
GDFSREELQQSQLEARVIAKKIKELIENRTEVYNTKTKSSRPVTYRDIVILLRSMTWAPEIMEEFKHQGIPVYANLSTGY
FEATEVSIMMSLLKVIDNPYQDIPLAAVLRSPIVGLNEEELSQIRISNKWGSFYEALMSFCQAKPEADQEALYEKVRPFT
EQLQYWRTKARQGSISELIWDLFRETKFYDFVGGMPGGKQRQANLRALYDRARQYESTSFRGLFRFLRFIERMIDRGDDL
GAARALGEQEDVVRIMTIHSSKGLEFPVVFIAGLARKFNTMDLKKTFMLDKEFGFAARYVNAEKRISYPTLAQLSFKRKK
KMEMLAEEMRVLYVALTRAKEKLFLIGSVKNAEKSLDRWLSAAATENWLLNDYERASANSYLDWIGPALIRHKDCVVLRA
GINQEKIKLLDHPSCWHVEMIHSEEAGALIEDKRNEKEELLEKVAEGKQVPIISKYAERVNEQLSWKYEFHSASVFRFKQ
SVSEIKRQYDLAGEESSTELLRKIRKPLLKRPRFMQEKTLTPAEKGTAMHMVMQQIDLSIPLTEESIRHQVDAMVQKELL
TAEQREGIDPELVLKFFKSDLGQRMLRAEKVMREVPFSLSIPAKETYTDWTGGDEQVLIQGVIDCFFEEEEGIILLDYKT
DRITDRYKGGFEEAKPILESRYSLQINFYARALEQIVRKPVYERYLFFFDGGHLLKLENN

Nucleotide


Download         Length: 3783 bp        

>NTDB_id=267504 C0971_RS04485 WP_248893055.1 879758..883540(+) (addA) [Bacillus methanolicus strain DFS2]
ATGATCTTGACAAATTCGGTGATAATTCCGCCAAAGCCTGAAAATGTGACATGGACCGATGACCAATGGAAAGCAATAAT
GGCAAAAGACCGGGACATCCTTGTTGCGGCGGCTGCCGGTTCGGGAAAAACGGCCGTTCTCGTTGAGAGAATCATTCGAA
GAATTACTTCCTTGCATGACCCGATTGATGTTGACCAGCTGCTAGTCGTTACTTTTACGAATGCTTCAGCTGCTGAAATG
AGACATCGAATTGGTGAAGCATTAGAAAAAGAGATTAATCAAAACCCAACCTCTGCGCATTTGCGTAAACAATTGAGTCT
GTTGAATAAAGCATCGATTTCAACGCTCCATTCCTTTTGCTTGGAAGTGATAAAAAAATACTATTATTTAATTGATATTG
ATCCAGGGTTCCGGATTGCCGATGAGACGGAAGCTCAACTTATGCGTGACGAGGTTTTGGATGAATTATTTGAAGAAGAA
TACGGTAAAAGTGATAATGAAGCATTCTTTTCACTTGTTGATACGTTTACGAATGACCGGAGCGATGAAGCCCTGCAAGA
TATTATCAGGGAACTTTATGATTTTGCCCGTTCTAATCCTTCTCCTGACCGTTATTTGAAATCGATTATCGAAATGTATG
ATGTTGATGAGAATGGGAAATTAGAAGATCTTCCATTTATGGATGCTCTTTTAGATGACATAGAGATGCAGCTTGAAGGG
GCAAAGCAAATGCTGCTTGAAGGGCTGGAGCTGACGAAGCTTCCCGGAGGACCTGCACCTAGAGCAGAAAATTTTATGGA
TGACTTGCATATTGTTGATACGCTCATTAGCGCAAAAAACGATTCATGGTCAACATTGTATAACGCAATGCAAAACCTGT
CCTTTTCACGTGCTAAAACAGTTCGAGGAGACGAATATGACGGTGATCTGGTCGAAAAAGCCCAAAAATTGAGAGAAAGG
GCGAAAAAAGTTTTGCAGGATTTACAGTCCGAACTTTTCTCAAGAAGGCCTGAATCGTTTTTAAAAGATATGCGAGAAAT
GAAAGGACATATTGAAACTCTCATTTCACTTGTGAAATCTTTTTCGGATCGATTTGAACAAGTGAAAAGAGAAAAGGGAT
TAGTTGATTTTGCTGATTTAGAGCATTACTGTCTTGATATTCTAACCGGAAGCATTGATGAAGACGGAAGAAGAATTCCT
TCCGAAGCAGCACTTTCTTACCGCCGTCTTTTTAAAGAAGTTCTTGTCGATGAGTACCAAGATACAAACATGGTGCAGGA
AGCCATATTGCAGCTCGTCACGAATGAAGAAGAACAGACAGGAAACCTCTTTATGGTAGGGGACGTCAAGCAATCGATCT
ACCGGTTCAGGCTGGCAGAGCCAAATTTATTTTTAAGCAAGTACAACCGTTTTAAGCCGACTGGTGCGGATTCAGGCTTG
AGAATCGATTTAGCCCGGAATTTCCGAAGCCGGAAAGAAGTGCTCGACGGAACGAATTTTCTGTTTAAGCAGTTTATGGG
GGTTAAGGTTGGAGAAATCGATTATGATGAAGCTGCAGAGTTAAAAAAAGGCGCTCCGTATCCTGAAGAAACATCAAACC
CTGTTTCCGTCATCTTGATCGACCAAGCCGGAGAGGATGACGAAAGCGATGCTGATGAATTGCCTGAAGCGGACAACATA
GGAGATTTCAGCCGGGAGGAATTGCAGCAGTCGCAGCTTGAAGCAAGGGTAATAGCAAAAAAAATCAAAGAGCTCATTGA
AAATCGGACTGAAGTATATAACACAAAAACAAAAAGCTCAAGGCCAGTTACTTACAGAGACATTGTAATTCTTCTCAGGT
CCATGACTTGGGCCCCAGAGATCATGGAAGAGTTTAAACACCAAGGTATACCGGTTTATGCAAATTTATCAACTGGATAT
TTTGAAGCAACAGAAGTATCAATTATGATGTCTTTGTTAAAAGTCATTGACAATCCTTATCAAGATATTCCGCTTGCAGC
CGTTTTACGCTCTCCGATTGTAGGATTAAACGAAGAAGAATTGTCGCAAATACGTATTTCAAATAAGTGGGGATCGTTCT
ATGAGGCTCTGATGTCTTTTTGTCAGGCAAAGCCGGAAGCAGATCAAGAAGCACTCTATGAAAAAGTCAGGCCGTTCACA
GAGCAGCTTCAATATTGGAGAACAAAAGCCCGTCAAGGATCAATATCTGAATTGATTTGGGATTTATTTAGGGAAACGAA
ATTTTATGATTTTGTCGGAGGGATGCCAGGCGGAAAACAGCGACAGGCCAACCTTAGAGCGTTATATGACAGGGCACGGC
AATATGAATCGACATCATTTAGAGGTTTGTTTCGCTTTCTCCGGTTTATAGAGCGCATGATCGACCGTGGCGATGACCTG
GGAGCAGCCCGTGCACTGGGAGAACAAGAAGATGTTGTAAGAATTATGACGATTCACAGCAGTAAAGGTCTTGAATTCCC
TGTCGTTTTTATTGCCGGTTTAGCGCGAAAATTCAATACAATGGATCTGAAAAAAACATTCATGCTGGATAAGGAATTTG
GATTCGCTGCCAGGTATGTAAATGCTGAAAAACGGATATCATACCCAACGCTTGCGCAGCTTTCATTTAAGCGTAAAAAG
AAAATGGAAATGCTGGCAGAGGAAATGAGAGTGTTATATGTTGCTTTAACCCGTGCAAAAGAGAAGCTGTTTTTGATAGG
ATCGGTAAAAAATGCTGAAAAATCATTGGACAGATGGCTCTCGGCAGCTGCCACCGAAAACTGGCTATTAAATGATTATG
AGCGGGCGAGTGCAAATAGCTATTTGGATTGGATCGGCCCTGCGCTTATTAGACATAAGGATTGTGTAGTTTTGCGAGCT
GGCATTAATCAGGAAAAAATAAAACTATTAGACCACCCTTCATGCTGGCATGTTGAAATGATTCATAGTGAAGAAGCTGG
AGCATTGATTGAAGATAAAAGAAACGAAAAAGAAGAATTGTTGGAAAAAGTAGCTGAAGGCAAACAGGTTCCAATTATTT
CTAAATATGCAGAACGGGTAAACGAACAGCTTTCATGGAAATATGAATTTCATAGTGCTTCTGTGTTCCGGTTCAAGCAA
TCTGTTTCTGAGATTAAGAGGCAGTATGATCTTGCCGGTGAAGAGAGCAGTACGGAACTGCTTCGGAAAATAAGAAAGCC
TCTTTTAAAGCGGCCAAGATTTATGCAAGAAAAAACGTTAACACCAGCAGAAAAAGGAACGGCAATGCATATGGTAATGC
AGCAGATTGATCTGTCGATTCCTTTAACGGAAGAATCGATCCGTCATCAGGTAGATGCCATGGTACAGAAAGAACTCCTT
ACCGCGGAGCAAAGAGAAGGAATCGATCCTGAACTTGTTTTGAAATTTTTTAAGAGCGATCTTGGGCAAAGGATGCTGCG
TGCAGAAAAGGTTATGCGCGAGGTTCCTTTCAGTCTATCAATTCCGGCAAAAGAAACGTATACCGATTGGACTGGTGGAG
ATGAACAAGTCCTTATACAAGGTGTGATTGACTGTTTCTTTGAAGAAGAGGAAGGAATTATACTCCTTGACTATAAAACA
GATCGTATTACGGATCGTTACAAAGGGGGATTTGAAGAAGCAAAACCAATTCTTGAATCGAGGTACAGCCTTCAAATAAA
CTTTTATGCCCGTGCACTTGAACAAATAGTAAGAAAGCCTGTCTATGAGAGATATTTGTTCTTCTTTGACGGGGGACATT
TATTAAAGCTAGAAAATAATTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(18-472)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1073-1238)

UvrD-like helicase C-terminal domain (UvrD_C)

(482-907)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

57.28

99.206

0.568

Multiple sequence alignment