Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   C0971_RS04480 Genome accession   NZ_CP026143
Coordinates   876274..879798 (+) Length   1174 a.a.
NCBI ID   WP_248893052.1    Uniprot ID   -
Organism   Bacillus methanolicus strain DFS2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 871274..884798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS04450 (C0971_04505) - 871933..872169 (-) 237 WP_004433464.1 IDEAL domain-containing protein -
  C0971_RS04455 (C0971_04510) - 872362..872940 (+) 579 WP_004433465.1 competence protein ComK -
  C0971_RS04460 (C0971_04515) - 872972..873403 (-) 432 WP_004433467.1 hypothetical protein -
  C0971_RS04465 (C0971_04525) - 873741..874676 (+) 936 WP_248893046.1 LCP family protein -
  C0971_RS04470 (C0971_04530) - 874787..875398 (+) 612 WP_248893048.1 TVP38/TMEM64 family protein -
  C0971_RS04475 (C0971_04535) lepB 875436..875990 (+) 555 WP_248893050.1 signal peptidase I -
  C0971_RS04480 (C0971_04540) addB 876274..879798 (+) 3525 WP_248893052.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  C0971_RS04485 (C0971_04545) addA 879758..883540 (+) 3783 WP_248893055.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  C0971_RS04490 (C0971_04550) - 883583..883804 (-) 222 WP_004433484.1 spore germination protein -
  C0971_RS04495 (C0971_04555) - 883870..884259 (-) 390 WP_248893057.1 spore germination protein GerPE -
  C0971_RS04500 (C0971_04560) - 884261..884443 (-) 183 WP_004433491.1 hypothetical protein -

Sequence

Protein


Download         Length: 1174 a.a.        Molecular weight: 135468.98 Da        Isoelectric Point: 6.7690

>NTDB_id=267503 C0971_RS04480 WP_248893052.1 876274..879798(+) (addB) [Bacillus methanolicus strain DFS2]
MAVRLITGRSGSGKTEYCLKEIREKLQQNPKGNPIIYLVPEQMTFLSEYKLVTTPNLGGMIRAQVFSFTRLAWRILQETG
GISRYHLNSVGINMLIRKIIEDQKDELKIFQRAADKSGFIQQMEQILTEFKRYCVTPGELLEKQSAFWPDEEQNYKDLKD
KLHDLELIYSRFEDELFGKYVDSEDYLRLLSEKIASSKYLKEADIYIDGFYTYTPQEYMIIEKLIKHCKRVTITLTSDKL
YKENAPDELHLFRLAGETSLTIYEIIKTNGIELEEVVHLEEQLRWTNASLRHLESEFDTRPAVPYQGTPEISICQAVNRR
AEIEGVARKIKYLVREKGYRFRDIALLVRNGSDYYDILETIFDDHEIPYFIDQKRTMLNHPLIELIRSSLEVINGHWRYE
PVFRAIKTELMFPVKSNVFKLRQQMDRLENYVLAYGIQGDKWTKKERWIYRRIRGLEFEAVAKTDAEKNIEQELNELRAM
VTAPLLRMARRLKKAETGRDLCEAVYLFLEELDIPAKLEQWKLAAEVNGELVKAREHDQAWNAVMELLDQFVEMLGEEKI
TLKQFASILDAGMESLRFSLVPPAIDQVLVADLERSRLSDIKAAFVIGLNEGVFPAKMGEEGILADEDREILLSKGLKIA
PGSRTRLLDENFLAYKAFVTPSEHLYVSYPLANEEGKALMPSSYIKRITDLFPNARMHFFVADPSELSEEEQLEFISNET
TALSYLTGQLQLKKRNYPVYDLWWDVYNYYINSDRLRPAAIKALSGLFYENRTKKLSEQVTKKLYGDHIQASVSRMELFH
SCPFSHFARHGLKLAERQVFRLEAPDIGKLFHAALKYIAETVTQKNLSWANMTKDQCEILAREAVEKLAPKLQNEILLST
NRHQYIKRKLEKIISRASFVLSEHAKASGFSPIGLEVAFGPKEKLPPLSFSLKNGTKMELVGRIDRVDKAEDTSGVFLRV
IDYKSSEKDLNIGEVYYGLALQMLTYLDIIITHSDNLIGTKADPAGVLYFHVHNPIIHSSKVLTLDEIEKEIMKSFRMKG
LLLGEQNVIQLMDKTLESGDSQIVSAGMKKDGTLKKSSKVATREEFEALRRYVRKMYEKTGNAITEGIVDLSPYKMKNKT
PCTFCPYKAVCQFDQSLESNEFRILTPYKKDDVLDLIRKEVDDLDKFGDNSAKA

Nucleotide


Download         Length: 3525 bp        

>NTDB_id=267503 C0971_RS04480 WP_248893052.1 876274..879798(+) (addB) [Bacillus methanolicus strain DFS2]
ATGGCTGTAAGACTCATAACAGGGAGATCAGGAAGCGGCAAAACTGAATATTGTTTAAAGGAAATACGGGAGAAACTGCA
GCAGAATCCGAAAGGAAACCCGATTATTTATCTCGTTCCTGAGCAGATGACATTTTTATCTGAATACAAACTTGTCACTA
CCCCTAATCTGGGCGGCATGATTCGTGCCCAAGTATTCAGTTTTACCCGTTTGGCCTGGAGAATTCTTCAGGAAACAGGC
GGGATCAGCCGTTATCATCTAAACAGTGTTGGGATTAACATGCTCATCAGAAAAATTATTGAAGATCAGAAGGATGAGTT
GAAAATCTTCCAACGGGCAGCTGATAAATCCGGATTTATCCAGCAAATGGAACAAATACTGACGGAATTTAAGCGATATT
GTGTAACACCAGGAGAACTTTTGGAGAAACAATCCGCGTTTTGGCCGGATGAGGAACAAAATTACAAGGATCTAAAGGAT
AAGCTTCACGATCTTGAATTAATTTACAGCCGATTTGAGGATGAACTGTTTGGGAAATATGTAGATTCCGAGGATTACCT
CAGGCTTCTTTCTGAAAAAATCGCTTCATCCAAGTATTTAAAAGAAGCAGATATTTATATCGACGGTTTTTACACTTACA
CCCCGCAGGAGTATATGATTATCGAAAAATTAATAAAGCATTGCAAGCGGGTTACGATTACTTTAACAAGTGACAAATTG
TATAAAGAAAACGCTCCTGATGAGCTTCATCTTTTCAGATTGGCCGGTGAAACGAGCTTGACGATTTATGAAATCATCAA
AACAAATGGGATCGAATTAGAAGAAGTAGTTCATCTTGAGGAGCAGCTGAGGTGGACAAACGCTTCATTGCGCCATTTAG
AATCAGAATTTGATACAAGGCCTGCCGTACCTTATCAGGGCACACCTGAGATTTCGATTTGCCAAGCGGTTAACAGGCGG
GCGGAAATAGAAGGAGTTGCCCGAAAGATCAAGTATTTAGTGAGAGAAAAAGGATACCGGTTCCGGGATATCGCGTTGTT
GGTAAGGAATGGAAGTGATTATTACGATATTTTAGAGACAATTTTTGATGATCATGAAATACCATATTTTATTGATCAGA
AACGAACAATGTTGAACCATCCGCTCATTGAGTTAATACGCTCCAGTCTGGAAGTCATTAATGGCCATTGGCGTTATGAA
CCGGTATTTCGCGCCATAAAAACAGAGCTAATGTTTCCTGTAAAATCCAATGTTTTTAAGTTGCGCCAGCAGATGGACAG
GCTTGAAAATTACGTTCTCGCTTACGGAATTCAAGGAGATAAATGGACAAAAAAAGAGCGCTGGATCTACCGTAGAATAA
GAGGGCTTGAGTTTGAAGCAGTTGCAAAGACCGATGCAGAAAAGAACATTGAACAAGAACTAAATGAGCTCAGGGCTATG
GTAACTGCCCCGCTTCTTCGGATGGCCCGCCGTCTTAAAAAAGCTGAAACTGGAAGAGATTTATGCGAAGCTGTCTATTT
ATTTTTGGAAGAATTAGATATCCCCGCAAAACTTGAACAATGGAAGCTTGCTGCTGAAGTAAATGGCGAGCTAGTAAAAG
CCCGGGAGCATGATCAAGCCTGGAACGCCGTAATGGAGTTGTTGGATCAGTTTGTTGAAATGCTTGGTGAGGAAAAAATT
ACACTAAAGCAGTTTGCATCTATTCTTGATGCCGGGATGGAGTCATTGCGATTTTCACTTGTTCCTCCTGCTATCGACCA
AGTTTTAGTTGCAGATTTGGAGAGATCAAGGCTGTCAGATATTAAAGCAGCTTTTGTCATCGGTTTAAATGAAGGGGTTT
TTCCTGCAAAAATGGGTGAGGAAGGAATTCTAGCTGACGAAGACCGTGAGATTTTGCTGTCAAAGGGATTGAAAATAGCG
CCTGGAAGCCGTACGCGCCTTTTGGATGAAAACTTTCTCGCCTATAAAGCATTTGTTACTCCTTCCGAGCACCTTTATGT
AAGTTATCCTTTGGCAAATGAAGAAGGGAAAGCATTGATGCCGTCTTCATATATTAAAAGAATAACGGATCTGTTTCCAA
ATGCACGCATGCATTTTTTTGTTGCCGATCCATCAGAGTTATCGGAAGAAGAGCAGCTTGAATTTATATCAAATGAAACG
ACTGCCCTGTCTTATTTGACCGGACAACTCCAACTGAAAAAACGCAATTATCCGGTTTATGATTTATGGTGGGATGTTTA
TAACTATTATATAAATAGTGACAGATTAAGGCCGGCTGCAATTAAAGCTCTGTCTGGCTTATTCTATGAAAACCGGACGA
AAAAGTTATCAGAACAGGTAACTAAAAAATTATACGGAGATCATATTCAAGCAAGCGTGTCAAGGATGGAGCTCTTTCAC
AGCTGTCCGTTCTCCCATTTTGCTCGACATGGTTTAAAACTGGCGGAACGGCAGGTATTTCGTCTTGAAGCACCTGATAT
CGGTAAATTATTCCATGCTGCATTAAAGTATATCGCAGAAACAGTGACGCAAAAAAATTTGTCCTGGGCAAACATGACAA
AGGATCAATGTGAAATTCTTGCGAGAGAAGCGGTTGAAAAACTGGCTCCCAAATTGCAAAATGAAATCCTGTTGAGTACA
AATCGCCATCAATACATTAAAAGAAAACTTGAAAAAATTATTAGCAGGGCTTCGTTTGTATTAAGTGAACATGCAAAGGC
AAGCGGTTTTTCCCCGATAGGCTTGGAAGTGGCTTTCGGTCCGAAGGAAAAACTGCCGCCTCTATCATTCTCTTTAAAAA
ATGGAACGAAAATGGAATTGGTTGGCCGGATTGACCGGGTTGATAAAGCAGAAGACACGAGCGGCGTATTTTTAAGAGTT
ATAGATTACAAGTCGAGCGAAAAAGATTTAAATATTGGGGAAGTTTACTACGGTCTTGCACTGCAAATGCTTACGTACCT
TGATATTATCATTACCCATTCGGATAATTTAATTGGTACGAAAGCAGACCCTGCGGGAGTCCTATATTTCCATGTTCATA
ATCCGATCATCCATTCTTCAAAAGTGCTTACGCTTGATGAAATTGAAAAAGAGATTATGAAAAGCTTTCGAATGAAAGGG
CTTCTTCTCGGGGAGCAAAATGTAATTCAATTAATGGACAAAACGCTGGAATCCGGAGACTCGCAAATTGTCTCCGCCGG
AATGAAAAAGGATGGAACGTTGAAAAAGTCTTCGAAAGTGGCGACAAGAGAAGAGTTCGAAGCATTAAGGCGTTATGTCC
GGAAAATGTATGAGAAAACAGGCAATGCCATTACAGAGGGAATAGTCGACCTTTCTCCTTATAAAATGAAGAACAAAACT
CCGTGCACGTTTTGTCCGTATAAAGCGGTGTGCCAGTTCGATCAATCACTAGAAAGCAATGAGTTTCGTATTTTAACGCC
ATACAAAAAAGATGATGTTTTGGATTTAATTCGAAAGGAGGTAGATGATCTTGACAAATTCGGTGATAATTCCGCCAAAG
CCTGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(286-628)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

51.887

99.319

0.515

Multiple sequence alignment