Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C0971_RS00565 Genome accession   NZ_CP026143
Coordinates   108861..110240 (+) Length   459 a.a.
NCBI ID   WP_248892619.1    Uniprot ID   -
Organism   Bacillus methanolicus strain DFS2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 103861..115240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS00545 (C0971_00540) - 104156..104614 (+) 459 WP_003348674.1 CtsR family transcriptional regulator -
  C0971_RS00550 (C0971_00545) - 104641..105189 (+) 549 WP_248893921.1 UvrB/UvrC motif-containing protein -
  C0971_RS00555 (C0971_00550) - 105192..106256 (+) 1065 WP_248892617.1 protein arginine kinase -
  C0971_RS00560 (C0971_00555) clpC 106279..108723 (+) 2445 WP_248892618.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  C0971_RS00565 (C0971_00560) radA 108861..110240 (+) 1380 WP_248892619.1 DNA repair protein RadA Machinery gene
  C0971_RS00570 (C0971_00565) disA 110243..111316 (+) 1074 WP_248892620.1 DNA integrity scanning diadenylate cyclase DisA -
  C0971_RS00575 (C0971_00570) - 111537..112619 (+) 1083 WP_003348663.1 PIN/TRAM domain-containing protein -
  C0971_RS00580 (C0971_00575) ispD 112686..113384 (+) 699 WP_003348661.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C0971_RS00585 (C0971_00580) ispF 113415..113894 (+) 480 WP_003348659.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49819.33 Da        Isoelectric Point: 8.2303

>NTDB_id=267499 C0971_RS00565 WP_248892619.1 108861..110240(+) (radA) [Bacillus methanolicus strain DFS2]
MAKRKTKFICQKCGYESPKWMGKCPGCGEWNKMVEEVEPASTGRRGAFAYSVGTSSSSKATPITSIETLSEPRIQTDLNE
FNRVLGSGIVRGSLVLIGGDPGIGKSTLLLQVSSQLAAKSYQVLYVSGEESLRQLKLRANRLGVSSENLLVYSETNLEEI
SKTIENVSPSFVIVDSIQTVFHPEITSAPGSVSQVRECTAELMRIAKSKGIAIFIVGHVTKEGSIAGPRILEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPSS
FGIPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGIKLDEPAIDLAIAISIASSFRGKPTRPTDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPQNNLGGWKGPKGIELVGVSSVSQALQVTLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=267499 C0971_RS00565 WP_248892619.1 108861..110240(+) (radA) [Bacillus methanolicus strain DFS2]
ATGGCAAAACGAAAAACGAAGTTTATATGCCAGAAATGCGGCTATGAATCGCCGAAATGGATGGGCAAATGCCCGGGCTG
CGGTGAGTGGAACAAAATGGTTGAGGAAGTAGAACCGGCCAGCACAGGGAGAAGAGGGGCCTTCGCATACTCTGTCGGAA
CTTCATCAAGTTCAAAAGCTACGCCGATTACATCAATTGAAACCTTAAGTGAACCGCGAATTCAAACAGATCTAAATGAA
TTCAATAGGGTTCTTGGAAGTGGGATCGTCAGAGGTTCTTTAGTATTAATCGGAGGGGATCCTGGAATAGGAAAATCAAC
ATTGCTTTTACAAGTATCTTCACAGCTTGCTGCCAAAAGTTATCAAGTTTTATATGTATCCGGAGAGGAATCGCTGCGCC
AGCTAAAGCTTAGGGCGAATCGCCTTGGAGTGTCTTCTGAAAATTTGCTTGTGTATTCAGAAACCAATCTTGAAGAAATA
AGCAAAACGATTGAAAATGTTTCACCAAGCTTTGTTATTGTTGACTCAATCCAAACGGTTTTCCACCCCGAAATTACTTC
AGCACCTGGAAGCGTTTCGCAAGTAAGAGAGTGTACGGCAGAATTGATGAGAATTGCGAAATCGAAAGGGATTGCTATAT
TTATTGTGGGGCATGTTACAAAAGAAGGATCGATTGCAGGTCCGCGCATTTTGGAACACATGGTTGATACCGTTTTATAT
TTTGAAGGAGAGAGGCACCATACGTACCGCATATTACGCGCAGTCAAAAACAGGTTCGGTTCGACAAATGAAATGGGTAT
TTTTGAGATGAAAGAATTTGGCCTTGAAGAAGTTGAAAATCCGTCGGAAATTTTTCTAGAAGAACGGTCTCAAGGTGCAG
CAGGTTCTACAGTGGTCGCTTCAATGGAAGGAACGAGACCGGTACTTGTGGAAATTCAAGCGCTTATTTCACCTTCGAGC
TTCGGAATCCCAAGGCGGATGGCAACAGGAATTGATCATAACAGGGTTCCGCTTTTAATGGCTGTTCTTGAGAAAAGAGT
CGGAATGCTGCTTCAAAACCAGGATGCGTATTTAAAAGTGGCTGGCGGAATAAAGCTTGACGAGCCAGCTATTGATCTGG
CAATAGCGATAAGCATTGCATCTAGTTTTCGTGGCAAGCCGACAAGACCAACGGATTGTATTATAGGCGAAGTCGGATTG
ACAGGCGAAGTAAGGCGAGTTTCAAGGATTGAGCAAAGGGTGCAGGAAGCAGCGAAACTTGGTTTTGAAAGAGTGATTTT
GCCGCAAAATAATCTCGGAGGATGGAAAGGTCCGAAAGGCATCGAATTGGTCGGAGTTTCATCTGTGAGCCAGGCACTTC
AAGTTACGTTAGGGGGATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(75-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.522

100

0.767
  radA Streptococcus pneumoniae Rx1

61.013

98.911

0.603
  radA Streptococcus pneumoniae D39

61.013

98.911

0.603
  radA Streptococcus pneumoniae R6

61.013

98.911

0.603
  radA Streptococcus pneumoniae TIGR4

61.013

98.911

0.603
  radA Streptococcus mitis NCTC 12261

61.013

98.911

0.603
  radA Streptococcus mitis SK321

60.793

98.911

0.601

Multiple sequence alignment