Detailed information NTG0267399

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C2U32_RS20650
• Genome accession: NZ_CP026125
• Coordinates: 3301465..3302325 (-)
• Length: 286 a.a.
• NCBI ID: WP_002061654.1
• Uniprot ID: N9KL48
• Organism: Acinetobacter baumannii strain ABNIH28
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3296465..3307325

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2U32_RS16255 (C2U32_16245)	-	3297356..3298666 (+)	1311	WP_004842687.1	tetratricopeptide repeat protein	-
C2U32_RS16260 (C2U32_16250)	-	3298743..3299069 (+)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
C2U32_RS16265 (C2U32_16255)	rlmB	3299171..3299920 (+)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
C2U32_RS16270 (C2U32_16260)	-	3299956..3300870 (+)	915	WP_000075011.1	DMT family transporter	-
C2U32_RS16275 (C2U32_16265)	coaE	3300867..3301463 (-)	597	WP_000874125.1	dephospho-CoA kinase	-
C2U32_RS20650 (C2U32_16270)	pilD	3301465..3302325 (-)	861	WP_002061654.1	prepilin peptidase	Machinery gene
C2U32_RS16285 (C2U32_16275)	pilC	3302325..3303551 (-)	1227	WP_000279218.1	type II secretion system F family protein	Machinery gene
C2U32_RS16290 (C2U32_16280)	pilB	3303581..3305293 (-)	1713	WP_002058663.1	type IV-A pilus assembly ATPase PilB	Machinery gene
C2U32_RS16295 (C2U32_16285)	tpiA	3305584..3306378 (+)	795	WP_000016939.1	triose-phosphate isomerase	-
C2U32_RS16300 (C2U32_16290)	secG	3306391..3306720 (+)	330	WP_000555570.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32083.45 Da
• Isoelectric Point: 7.1041

>NTDB_id=267399 C2U32_RS20650 WP_002061654.1 3301465..3302325(-) (pilD) [Acinetobacter baumannii strain ABNIH28]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHERLTLNKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMAFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=267399 C2U32_RS20650 WP_002061654.1 3301465..3302325(-) (pilD) [Acinetobacter baumannii strain ABNIH28]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACGCCAAAAATGATGGAACAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCCGAGCAACCGATTATTGATCATGAGAGGTTAACTTTAAATAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCAATCAG
TATTCGCTATCCAGCCATCGAACTACTCACCATGCTATGTTCATTAGTAGTAGTCATGGCGTTTGGCCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCTTACCTTTAGCTGCACTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGTGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

3D structure

• AlphaFold DB: N9KL48

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	99.65	100	0.997
pilD	Acinetobacter nosocomialis M2	97.902	100	0.979
pilD	Vibrio cholerae strain A1552	53.309	95.105	0.507
pilD	Vibrio campbellii strain DS40M4	52.399	94.755	0.497
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441