Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CXW77_RS10025 Genome accession   NZ_CP025910
Coordinates   2049733..2050713 (+) Length   326 a.a.
NCBI ID   WP_001055618.1    Uniprot ID   A0A7U9NYG1
Organism   Escherichia coli strain 204446     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2044733..2055713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXW77_RS09995 - 2045111..2046097 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  CXW77_RS10000 hemW 2046410..2047546 (-) 1137 WP_000239948.1 radical SAM family heme chaperone HemW -
  CXW77_RS10005 rdgB 2047539..2048132 (-) 594 WP_001174744.1 XTP/dITP diphosphatase -
  CXW77_RS10010 yggU 2048140..2048430 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  CXW77_RS10015 yggT 2048427..2048993 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  CXW77_RS10020 yggS 2049011..2049715 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  CXW77_RS10025 pilT 2049733..2050713 (+) 981 WP_001055618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CXW77_RS10030 ruvX 2050889..2051305 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  CXW77_RS10035 yqgE 2051305..2051868 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  CXW77_RS10040 gshB 2051977..2052927 (-) 951 WP_000593273.1 glutathione synthase -
  CXW77_RS10045 rsmE 2052940..2053671 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CXW77_RS10050 endA 2053751..2054458 (-) 708 WP_000286500.1 deoxyribonuclease I -
  CXW77_RS10055 yggI 2054553..2055050 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35899.09 Da        Isoelectric Point: 5.5860

>NTDB_id=264412 CXW77_RS10025 WP_001055618.1 2049733..2050713(+) (pilT) [Escherichia coli strain 204446]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=264412 CXW77_RS10025 WP_001055618.1 2049733..2050713(+) (pilT) [Escherichia coli strain 204446]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U9NYG1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment