Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C1H71_RS09585 Genome accession   NZ_CP025781
Coordinates   2130479..2131855 (+) Length   458 a.a.
NCBI ID   WP_130106360.1    Uniprot ID   A0A7G3G8J7
Organism   Iodobacter fluviatilis strain PCH194     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2125479..2136855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1H71_RS09560 (C1H71_09650) - 2127046..2128017 (+) 972 WP_130106355.1 KpsF/GutQ family sugar-phosphate isomerase -
  C1H71_RS09565 (C1H71_09655) kdsC 2128185..2128700 (+) 516 WP_130106356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  C1H71_RS09570 (C1H71_09660) lptC 2128700..2129278 (+) 579 WP_130106357.1 LPS export ABC transporter periplasmic protein LptC -
  C1H71_RS09575 (C1H71_09665) lptA 2129256..2129756 (+) 501 WP_130106358.1 lipopolysaccharide transport periplasmic protein LptA -
  C1H71_RS09580 (C1H71_09670) lptB 2129753..2130478 (+) 726 WP_130106359.1 LPS export ABC transporter ATP-binding protein -
  C1H71_RS09585 (C1H71_09675) rpoN/rpoN1 2130479..2131855 (+) 1377 WP_130106360.1 RNA polymerase factor sigma-54 Machinery gene
  C1H71_RS09590 (C1H71_09680) hpf 2131880..2132206 (+) 327 WP_130106361.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  C1H71_RS09595 (C1H71_09685) - 2132312..2132785 (+) 474 WP_130106362.1 PTS sugar transporter subunit IIA -
  C1H71_RS09600 (C1H71_09690) hprK 2132766..2133710 (+) 945 WP_130106363.1 HPr(Ser) kinase/phosphatase -
  C1H71_RS09605 (C1H71_09695) rapZ 2133707..2134555 (+) 849 WP_130106364.1 RNase adapter RapZ -
  C1H71_RS09610 (C1H71_09700) - 2134629..2134988 (+) 360 WP_188053696.1 NusG domain II-containing protein -
  C1H71_RS09615 (C1H71_09705) - 2134963..2135487 (+) 525 WP_130106365.1 Gx transporter family protein -
  C1H71_RS09620 (C1H71_09710) - 2135703..2136311 (+) 609 WP_130106366.1 flavin prenyltransferase UbiX -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 51923.09 Da        Isoelectric Point: 4.8961

>NTDB_id=263748 C1H71_RS09585 WP_130106360.1 2130479..2131855(+) (rpoN/rpoN1) [Iodobacter fluviatilis strain PCH194]
MKQTLQLRLSQQLNLTPQLQQAIKLLQLSTLEFQQELEQYLNDNPLLERGEEAPAGEAEQPEPQWEMGGDDDGLRWEGTS
HGSSDDDNDFDPATNIARDLDLREYLLQQAGLLQLPHRDKSLLLMLIEALDDDGFLSQPLDEVYSQLPEELIQDYELELD
DLKIALIRLQHLEPLGVGARDTADSLCLQLRQKQPSAAKDLALKIAQNSLDLLAGRDYTKLKRAFKCEDAELKAAQELIV
SLNPRPSAQWSVTSPRYVSPDVVVQKVRGVWRVRLHDASMPKLRVNRLYADILQKGDGAHLARELQEARWLVKSVQQRFD
TILRVATAIVERQRAFFEYGEVAMRPLVLRDIAEELDLHESTISRVTTQKYMLTPRGLYEFKYFFGSHVDTDAGGECSAT
AIKALIKQMVQQEDKKKPLSDSVLAEALSAQGIVVARRTVAKYREALQIYPVSQRKSL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=263748 C1H71_RS09585 WP_130106360.1 2130479..2131855(+) (rpoN/rpoN1) [Iodobacter fluviatilis strain PCH194]
ATGAAACAAACCCTGCAGCTGCGGCTTTCCCAGCAACTTAACCTCACGCCACAATTGCAGCAGGCTATTAAGCTGCTGCA
GCTTTCTACGCTTGAGTTTCAACAAGAGCTTGAACAGTATCTAAACGACAACCCCTTGCTGGAGCGTGGCGAGGAGGCCC
CCGCAGGGGAAGCTGAGCAGCCCGAGCCGCAGTGGGAAATGGGCGGGGATGATGATGGTTTGCGCTGGGAAGGTACTAGT
CATGGAAGCTCAGATGACGATAATGACTTCGACCCCGCCACCAATATTGCGCGTGATTTAGATTTACGTGAATACCTGCT
GCAGCAAGCAGGTCTGCTGCAGCTGCCGCATCGGGATAAGTCTTTGTTGTTGATGTTGATTGAAGCTTTGGATGATGATG
GTTTTCTTTCTCAGCCCTTAGATGAAGTCTATTCTCAGCTGCCAGAGGAACTTATCCAAGATTATGAACTCGAACTTGAT
GACTTGAAGATTGCTTTAATTCGCTTACAACATTTAGAGCCCTTAGGCGTTGGAGCTCGGGATACCGCCGATTCATTATG
CTTACAGTTGCGTCAAAAACAGCCGAGTGCCGCTAAAGATTTAGCCTTGAAAATTGCCCAAAATTCACTCGATTTATTAG
CTGGACGGGACTACACTAAGCTTAAACGAGCCTTTAAATGCGAGGATGCTGAGTTAAAAGCTGCGCAAGAATTGATTGTA
AGTTTAAACCCAAGGCCGAGTGCACAGTGGTCTGTAACCTCACCAAGATATGTAAGCCCTGATGTGGTTGTGCAAAAAGT
ACGCGGGGTTTGGCGGGTTAGATTACACGATGCATCTATGCCAAAATTACGCGTTAACCGGCTTTATGCAGATATCTTAC
AAAAAGGCGATGGTGCTCATTTAGCGCGCGAGTTACAAGAAGCGCGTTGGTTGGTAAAAAGTGTGCAGCAGCGTTTTGAT
ACGATTTTACGAGTAGCAACAGCAATTGTAGAGCGGCAGCGGGCATTTTTTGAGTACGGTGAAGTGGCGATGCGCCCTTT
GGTGTTGAGGGATATCGCTGAAGAGCTCGATTTACACGAATCAACTATTTCTAGAGTCACCACGCAAAAATACATGTTAA
CGCCGCGTGGTTTGTATGAGTTTAAGTACTTTTTTGGTAGCCATGTAGATACGGACGCCGGTGGTGAATGTTCTGCAACC
GCTATCAAAGCTTTGATAAAGCAGATGGTGCAGCAAGAAGATAAGAAAAAACCTTTAAGTGATAGCGTATTGGCAGAAGC
ATTATCCGCCCAAGGTATTGTGGTAGCGCGTCGTACCGTTGCTAAATACCGTGAGGCGTTGCAGATTTACCCAGTAAGTC
AGCGCAAAAGTTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(301-456)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G3G8J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.779

100

0.572

Multiple sequence alignment