Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   Y31_RS16815 Genome accession   NZ_CP030910
Coordinates   3480519..3482240 (+) Length   573 a.a.
NCBI ID   WP_042158261.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Y31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3475519..3487240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Y31_RS16795 (Y31_3222) - 3476245..3477174 (-) 930 WP_003162124.1 LysR family transcriptional regulator -
  Y31_RS16800 (Y31_3223) - 3477197..3478171 (-) 975 WP_023116917.1 quinone oxidoreductase -
  Y31_RS16805 (Y31_3224) - 3478397..3479149 (+) 753 WP_115759567.1 class I SAM-dependent methyltransferase -
  Y31_RS16810 (Y31_3225) - 3479786..3480529 (+) 744 WP_115759865.1 ABC transporter permease -
  Y31_RS16815 (Y31_3226) pilF 3480519..3482240 (+) 1722 WP_042158261.1 GspE/PulE family protein Machinery gene
  Y31_RS16820 (Y31_3227) - 3482245..3483432 (+) 1188 WP_003097729.1 type II secretion system F family protein -
  Y31_RS16825 (Y31_3228) gspG 3483486..3483920 (+) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  Y31_RS16830 (Y31_3229) - 3483889..3484299 (+) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Y31_RS16835 (Y31_3230) - 3484299..3484724 (+) 426 WP_003116709.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Y31_RS16840 (Y31_3231) - 3484721..3485311 (+) 591 WP_003097720.1 type II secretion system protein -
  Y31_RS16845 (Y31_3232) - 3485301..3486380 (+) 1080 WP_003119955.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63499.57 Da        Isoelectric Point: 5.3299

>NTDB_id=261296 Y31_RS16815 WP_042158261.1 3480519..3482240(+) (pilF) [Pseudomonas aeruginosa strain Y31]
MSSEAVSPGPQMTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVHADIGLTFAAALRSILRQDPDVIMIGEIRD
LETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAATQ
AATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAWLG
MTSLDEVHRVTSG

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=261296 Y31_RS16815 WP_042158261.1 3480519..3482240(+) (pilF) [Pseudomonas aeruginosa strain Y31]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGATGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCACTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGTTACCTGCGGGAGTTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGTTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAAGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCATGCCGACA
TCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATACGCGAC
CTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGATGCGGT
GAGCGCCTTCACGCGTTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGGCCCAGC
GACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCGACCCAG
GCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGGTACCGG
CTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAGCTTCCC
TCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGGTTGGGT
ATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)

Type II/IV secretion system protein (T2SSE)

(194-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.519

98.778

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.624

98.953

0.382
  pilF Neisseria gonorrhoeae MS11

36.667

99.476

0.365