Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   CRH08_RS00820 Genome accession   NZ_CP025419
Coordinates   125619..126053 (+) Length   144 a.a.
NCBI ID   WP_009909097.1    Uniprot ID   -
Organism   Streptococcus suis strain SH0104     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120619..131053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRH08_RS00785 - 121361..121726 (+) 366 WP_002936568.1 DUF1033 family protein -
  CRH08_RS00790 - 121761..122711 (-) 951 WP_002936565.1 S66 peptidase family protein -
  CRH08_RS00795 comYA 122797..123747 (+) 951 WP_002936562.1 competence type IV pilus ATPase ComGA Machinery gene
  CRH08_RS00800 comYB 123659..124696 (+) 1038 WP_255195823.1 competence type IV pilus assembly protein ComGB Machinery gene
  CRH08_RS00805 comYC 124698..124979 (+) 282 WP_009909092.1 competence type IV pilus major pilin ComGC Machinery gene
  CRH08_RS00810 comGD 124960..125367 (+) 408 WP_009909093.1 competence type IV pilus minor pilin ComGD -
  CRH08_RS00815 comYE 125339..125632 (+) 294 WP_024390433.1 competence type IV pilus minor pilin ComGE Machinery gene
  CRH08_RS00820 comGF/cglF 125619..126053 (+) 435 WP_009909097.1 competence type IV pilus minor pilin ComGF Machinery gene
  CRH08_RS00825 comGG 126031..126441 (+) 411 WP_009909099.1 competence type IV pilus minor pilin ComGG -
  CRH08_RS00830 comYH 126498..127451 (+) 954 WP_024409299.1 class I SAM-dependent methyltransferase Machinery gene
  CRH08_RS00835 - 127501..128688 (+) 1188 WP_013730078.1 acetate kinase -
  CRH08_RS00845 - 129003..129599 (+) 597 WP_009909104.1 folate family ECF transporter S component -
  CRH08_RS00850 - 129610..130866 (+) 1257 WP_013730079.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16449.77 Da        Isoelectric Point: 5.9234

>NTDB_id=261099 CRH08_RS00820 WP_009909097.1 125619..126053(+) (comGF/cglF) [Streptococcus suis strain SH0104]
MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSWSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=261099 CRH08_RS00820 WP_009909097.1 125619..126053(+) (comGF/cglF) [Streptococcus suis strain SH0104]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCCTTAGTCATCCTTTCAGGTAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGCCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGTGAA
CAGGCTTTGGCCTTTGGCAAGTCATGGTCGGATGATTTTCGGAAAACAAATGATAGAGGCCAGGGTTACCAACCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

53.191

97.917

0.521
  comGF/cglF Streptococcus pneumoniae D39

53.191

97.917

0.521
  comGF/cglF Streptococcus pneumoniae R6

53.191

97.917

0.521
  comGF/cglF Streptococcus pneumoniae TIGR4

53.191

97.917

0.521
  comYF Streptococcus mutans UA140

50

100

0.5
  comGF/cglF Streptococcus mitis NCTC 12261

53.731

93.056

0.5
  comYF Streptococcus mutans UA159

49.306

100

0.493
  comGF/cglF Streptococcus mitis SK321

50.746

93.056

0.472
  comGF Lactococcus lactis subsp. cremoris KW2

46.809

97.917

0.458

Multiple sequence alignment