Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   CWI64_RS05360 Genome accession   NZ_CP025076
Coordinates   1000527..1001351 (-) Length   274 a.a.
NCBI ID   WP_001036779.1    Uniprot ID   P0A3S3
Organism   Streptococcus pneumoniae strain BHN97x     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 995527..1006351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI64_RS05355 (CWI64_05355) - 998967..1000298 (-) 1332 WP_000390047.1 hemolysin family protein -
  CWI64_RS05360 (CWI64_05360) endA 1000527..1001351 (-) 825 WP_001036779.1 DNA-entry nuclease EndA Machinery gene
  CWI64_RS05365 (CWI64_05365) - 1001390..1001578 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  CWI64_RS05370 (CWI64_05370) murA 1001571..1002854 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  CWI64_RS05375 (CWI64_05375) - 1002878..1003108 (-) 231 WP_000250384.1 DUF1146 family protein -
  CWI64_RS05380 (CWI64_05380) sepM 1003166..1004203 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  CWI64_RS05385 (CWI64_05385) coaD 1004187..1004675 (-) 489 WP_001280742.1 pantetheine-phosphate adenylyltransferase -
  CWI64_RS05390 (CWI64_05390) rsmD 1004665..1005204 (-) 540 WP_000706962.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CWI64_RS05395 (CWI64_05395) asnA 1005269..1006261 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29890.57 Da        Isoelectric Point: 10.1197

>NTDB_id=258084 CWI64_RS05360 WP_001036779.1 1000527..1001351(-) (endA) [Streptococcus pneumoniae strain BHN97x]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=258084 CWI64_RS05360 WP_001036779.1 1000527..1001351(-) (endA) [Streptococcus pneumoniae strain BHN97x]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCCGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGTAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3OWV

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

100

100

1
  endA Streptococcus pneumoniae D39

100

100

1
  endA Streptococcus pneumoniae R6

100

100

1
  endA Streptococcus pneumoniae TIGR4

100

100

1

Multiple sequence alignment