Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   CWI35_RS06095 Genome accession   NZ_CP025074
Coordinates   1159151..1160974 (-) Length   607 a.a.
NCBI ID   WP_013523174.1    Uniprot ID   -
Organism   [Bacillus] caldolyticus strain NEB414     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1154151..1165974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI35_RS06060 (CWI35_06050) - 1154618..1155256 (-) 639 WP_011230330.1 GTP pyrophosphokinase family protein -
  CWI35_RS06065 (CWI35_06055) - 1155394..1155771 (-) 378 WP_020278707.1 hypothetical protein -
  CWI35_RS06070 (CWI35_06060) - 1155907..1156500 (+) 594 WP_011230328.1 CYTH domain-containing protein -
  CWI35_RS06075 (CWI35_06065) - 1156497..1157120 (+) 624 WP_081132972.1 lytic transglycosylase domain-containing protein -
  CWI35_RS06080 (CWI35_06070) - 1157223..1157636 (+) 414 WP_011230326.1 hypothetical protein -
  CWI35_RS06085 (CWI35_06075) spxH 1157629..1158522 (+) 894 WP_011230325.1 ClpXP adapter protein SpxH -
  CWI35_RS06090 (CWI35_06080) - 1158940..1159137 (+) 198 WP_011230324.1 hypothetical protein -
  CWI35_RS06095 (CWI35_06085) pepF 1159151..1160974 (-) 1824 WP_013523174.1 oligoendopeptidase F Regulator
  CWI35_RS06100 (CWI35_06090) - 1160974..1162302 (-) 1329 WP_011230322.1 competence protein CoiA family protein -
  CWI35_RS06105 (CWI35_06095) cls 1162491..1163999 (-) 1509 WP_011230321.1 cardiolipin synthase -
  CWI35_RS06110 (CWI35_06100) mecA 1164096..1164776 (-) 681 WP_011230320.1 adaptor protein MecA Regulator
  CWI35_RS06115 (CWI35_06105) - 1164987..1165646 (+) 660 WP_014195176.1 TerC family protein -

Sequence

Protein


Download         Length: 607 a.a.        Molecular weight: 70330.41 Da        Isoelectric Point: 4.9959

>NTDB_id=258013 CWI35_RS06095 WP_013523174.1 1159151..1160974(-) (pepF) [[Bacillus] caldolyticus strain NEB414]
MTVEEKKAKKSLPLRSEIPVEETWRLEDIFPTDDAWEEEFKQVKAMIPKLGEYKGRLGESPEVLYEALQCQDDVSMRLGK
LYTYAHMRYDQDTTNSFYQGLDARAKSLYSEASSAMAFIVPEILAIDEAVLRSFLEQYEPLRLYAHALDEITRQRPHVLS
AEEEAILAQAAEVMQATSDTFSALNNADLTFPTIIDENGEEVEVTHGRFIRFLESTDRRVRRDAFYAVYHTYEKFQNTFA
NTLAGTVKKDNFFARIRRYRSAREAALDANNIPESVYDNLIATIHEHLPLLHRYVRLRKRVLGLDELHMYDLYTPLVQEV
KMEVTYEEAKQYMLEGLAPLGEEYVAIVKEGLDNRWVDVRENKGKRSGAYSSGAYGTHPYILLNWQDNVNNLFTLVHEFG
HSVHSYYTRKTQPYPYAHYSIFVAEVASTCNEALLNDYLLRTIDDEKKRLYLLNHYLEGFRGTVFRQTMFAEFEHLIHLK
AQQGEALTAETLTSLYYDLNKTYFGDDIVVDKEIGLEWARIPHFYYNYYVYQYATGFSAATALSKQILEEGEPAVRRYID
FLKAGSSDYPIEVLKKAGVDMTSAEPIRQACQVFAEKLDEMERLLEK

Nucleotide


Download         Length: 1824 bp        

>NTDB_id=258013 CWI35_RS06095 WP_013523174.1 1159151..1160974(-) (pepF) [[Bacillus] caldolyticus strain NEB414]
ATGACGGTGGAGGAAAAGAAAGCGAAAAAGTCGCTGCCGCTGCGAAGCGAGATTCCTGTGGAAGAAACGTGGCGGCTTGA
GGACATTTTTCCCACCGATGACGCCTGGGAAGAAGAATTCAAACAAGTGAAGGCAATGATTCCAAAGCTTGGCGAATACA
AAGGGCGGCTTGGCGAATCGCCGGAAGTGCTGTATGAAGCATTGCAATGCCAAGATGACGTATCGATGCGCCTCGGCAAG
CTGTATACATACGCCCATATGCGCTATGATCAAGATACGACTAACTCGTTTTACCAAGGGCTCGACGCCCGGGCGAAAAG
CTTGTACAGCGAAGCATCGAGCGCGATGGCGTTTATCGTGCCGGAAATTTTGGCGATCGATGAAGCGGTGCTGCGCTCGT
TTTTGGAACAATACGAGCCGCTTCGCTTGTATGCGCACGCGTTAGACGAAATTACGCGCCAGCGCCCGCACGTGCTGTCG
GCGGAAGAGGAGGCGATCTTGGCGCAGGCAGCCGAGGTGATGCAGGCGACATCCGACACGTTCAGCGCGTTGAACAACGC
TGATTTGACGTTTCCGACGATCATCGATGAAAACGGCGAGGAAGTCGAAGTCACGCACGGCCGGTTCATTCGCTTTTTAG
AAAGCACGGACCGCCGCGTCCGCCGCGATGCGTTTTACGCCGTATACCATACGTATGAGAAGTTTCAAAATACGTTCGCC
AATACGCTTGCCGGCACGGTGAAAAAAGACAACTTTTTCGCCCGCATCCGCCGCTACCGTTCGGCGCGGGAAGCGGCGTT
GGATGCAAACAACATTCCGGAGAGCGTCTATGACAACCTAATTGCTACGATTCATGAACATTTGCCGCTGTTGCATCGGT
ACGTGCGGCTGCGCAAACGAGTGCTTGGGCTTGATGAGCTGCATATGTACGATTTGTACACGCCGCTTGTGCAAGAAGTG
AAAATGGAAGTGACGTACGAGGAAGCGAAGCAATATATGCTTGAAGGGCTCGCGCCGCTTGGCGAGGAATATGTGGCGAT
CGTGAAAGAAGGTCTTGACAACCGTTGGGTCGATGTGCGCGAAAACAAAGGAAAGCGAAGCGGCGCCTATTCGTCGGGAG
CGTACGGCACCCATCCGTACATTTTGCTCAACTGGCAGGACAATGTGAACAATTTGTTTACACTTGTGCATGAGTTTGGC
CATTCGGTGCACAGCTACTATACGCGCAAAACGCAGCCGTATCCGTATGCGCATTATTCAATTTTCGTCGCCGAAGTGGC
GTCGACGTGCAATGAGGCGCTGTTGAACGACTATTTGCTGAGAACGATCGATGATGAGAAAAAGCGGCTCTATTTGCTCA
ACCATTATCTTGAGGGGTTCCGCGGCACGGTTTTTCGCCAGACGATGTTCGCGGAGTTTGAGCATTTGATCCATTTGAAG
GCGCAACAAGGCGAGGCATTGACGGCCGAGACGTTGACGTCCCTTTATTACGACTTAAATAAAACGTATTTTGGCGATGA
TATTGTCGTGGACAAAGAAATCGGCCTCGAGTGGGCGCGCATTCCGCATTTTTATTACAACTACTATGTGTACCAATACG
CAACCGGCTTCAGCGCGGCGACTGCACTTAGCAAACAAATTTTGGAAGAAGGCGAACCGGCGGTGAGACGGTACATCGAC
TTTTTAAAAGCCGGCAGTTCTGATTACCCGATTGAAGTATTGAAAAAAGCCGGCGTCGACATGACGAGCGCCGAGCCGAT
CCGCCAAGCGTGCCAAGTGTTTGCGGAGAAGCTTGATGAGATGGAGCGGCTGCTTGAGAAATGA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(122-191)

Peptidase family M3 (Peptidase_M3)

(213-592)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.265

97.694

0.501

Multiple sequence alignment