Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CWD84_RS21395 Genome accession   NZ_CP025001
Coordinates   4161449..4162828 (-) Length   459 a.a.
NCBI ID   WP_060963752.1    Uniprot ID   A0AAI8HS47
Organism   Bacillus siamensis strain SCSIO 05746     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4156449..4167828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWD84_RS21375 (CWD84_21370) ispF 4157968..4158444 (-) 477 WP_016935912.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  CWD84_RS21380 (CWD84_21375) ispD 4158437..4159135 (-) 699 WP_060965083.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CWD84_RS21385 (CWD84_21380) - 4159149..4160249 (-) 1101 WP_016935914.1 PIN/TRAM domain-containing protein -
  CWD84_RS21390 (CWD84_21385) disA 4160363..4161445 (-) 1083 WP_003156401.1 DNA integrity scanning diadenylate cyclase DisA -
  CWD84_RS21395 (CWD84_21390) radA 4161449..4162828 (-) 1380 WP_060963752.1 DNA repair protein RadA Machinery gene
  CWD84_RS21400 (CWD84_21395) clpC 4162922..4165357 (-) 2436 WP_060963753.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  CWD84_RS21405 (CWD84_21400) - 4165354..4166445 (-) 1092 WP_060963754.1 protein arginine kinase -
  CWD84_RS21410 (CWD84_21405) - 4166445..4167002 (-) 558 WP_016935918.1 UvrB/UvrC motif-containing protein -
  CWD84_RS21415 (CWD84_21410) ctsR 4167016..4167480 (-) 465 WP_016935919.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49487.82 Da        Isoelectric Point: 6.9565

>NTDB_id=257123 CWD84_RS21395 WP_060963752.1 4161449..4162828(-) (radA) [Bacillus siamensis strain SCSIO 05746]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETVKKEPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQLLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMVIPEANAGGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=257123 CWD84_RS21395 WP_060963752.1 4161449..4162828(-) (radA) [Bacillus siamensis strain SCSIO 05746]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGGAAATGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCGTCAAAAAAGAGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGTGTTCACACCAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGACCCCGGCATCGGGAAATCAACGCT
CCTTCTTCAAGTATCCGCACAATTAGCAGACACGGCCGGCAGCGTGCTTTACATTTCCGGGGAGGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGGCTTGGAATCAACAGCCAATTGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCCGCATTTGTTGTTGTAGACTCCATTCAGACCGTTTACCAAAGCGACATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCTGAATTGATGAAAATCGCGAAAACAAACGGCATTCCGATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCTGGGCCGAGATTGCTTGAGCATATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACAGAAGTGCTGAACCCTTCGGAAATTTTCTTGGAGGAGCGTTCTGCCGGGGCTTCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGCTTT
GGAAACCCGCGCCGCATGGCTACAGGGATTGACCACAACAGGGTGTCGTTAATCATGGCTGTGCTTGAGAAGCGGGTGGG
GCTATTGCTTCAGAATCAAGATGCGTACTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCGAATCCTGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAACTCGGCTTCAAGCGTATGGTCATACC
TGAGGCGAATGCAGGCGGATGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

92.795

99.782

0.926
  radA Streptococcus pneumoniae Rx1

64.018

98.693

0.632
  radA Streptococcus pneumoniae D39

64.018

98.693

0.632
  radA Streptococcus pneumoniae R6

64.018

98.693

0.632
  radA Streptococcus pneumoniae TIGR4

64.018

98.693

0.632
  radA Streptococcus mitis NCTC 12261

64.018

98.693

0.632
  radA Streptococcus mitis SK321

63.797

98.693

0.63

Multiple sequence alignment