Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CWD84_RS20205 Genome accession   NZ_CP025001
Coordinates   3940453..3940890 (+) Length   145 a.a.
NCBI ID   WP_060963635.1    Uniprot ID   A0AAI8N1S1
Organism   Bacillus siamensis strain SCSIO 05746     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3935453..3945890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWD84_RS20190 (CWD84_20185) - 3938311..3938673 (-) 363 WP_060963633.1 winged helix-turn-helix transcriptional regulator -
  CWD84_RS20195 (CWD84_20190) hxlA 3938906..3939541 (+) 636 WP_016936796.1 3-hexulose-6-phosphate synthase -
  CWD84_RS20200 (CWD84_20195) hxlB 3939538..3940095 (+) 558 WP_060963634.1 6-phospho-3-hexuloisomerase -
  CWD84_RS20205 (CWD84_20200) nucA/comI 3940453..3940890 (+) 438 WP_060963635.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CWD84_RS20210 (CWD84_20205) nin/comJ 3940912..3941310 (+) 399 WP_047476185.1 competence protein ComJ Regulator
  CWD84_RS20215 (CWD84_20210) - 3941470..3941740 (-) 271 Protein_3965 family 1 glycosylhydrolase -
  CWD84_RS20220 (CWD84_20215) - 3941841..3942119 (-) 279 WP_406795067.1 YckD family protein -
  CWD84_RS20225 (CWD84_20220) - 3942237..3942671 (-) 435 WP_060963638.1 RDD family protein -
  CWD84_RS20230 (CWD84_20225) - 3942688..3943824 (-) 1137 WP_060965076.1 zinc-dependent alcohol dehydrogenase -
  CWD84_RS20235 (CWD84_20230) - 3943888..3945090 (-) 1203 WP_060963639.1 GTP-binding protein -
  CWD84_RS22925 - 3945238..3945367 (+) 130 Protein_3970 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16082.39 Da        Isoelectric Point: 8.4850

>NTDB_id=257120 CWD84_RS20205 WP_060963635.1 3940453..3940890(+) (nucA/comI) [Bacillus siamensis strain SCSIO 05746]
MKTLKALLLVLIIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHPSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=257120 CWD84_RS20205 WP_060963635.1 3940453..3940890(+) (nucA/comI) [Bacillus siamensis strain SCSIO 05746]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTTGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAAAGCGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATCCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTAAAAACGGGGTACGACAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGGGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGATGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634

Multiple sequence alignment