Detailed information NTG0257108

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: CWD84_RS16340
• Genome accession: NZ_CP025001
• Coordinates: 3196879..3200583 (-)
• Length: 1234 a.a.
• NCBI ID: WP_060965056.1
• Uniprot ID: A0AAI8HSI3
• Organism: Bacillus siamensis strain SCSIO 05746
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3191879..3205583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWD84_RS16325 (CWD84_16325)	-	3191945..3192238 (-)	294	WP_017417418.1	hypothetical protein	-
CWD84_RS16330 (CWD84_16330)	sbcC	3192254..3195646 (-)	3393	WP_060963122.1	exonuclease subunit SbcC	-
CWD84_RS16335 (CWD84_16335)	sbcD	3195643..3196815 (-)	1173	WP_047476740.1	exonuclease subunit SbcD	-
CWD84_RS16340 (CWD84_16340)	addA	3196879..3200583 (-)	3705	WP_060965056.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
CWD84_RS16345 (CWD84_16345)	addB	3200570..3204070 (-)	3501	WP_060963123.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
CWD84_RS16350 (CWD84_16350)	-	3204215..3204925 (+)	711	WP_060963124.1	DUF421 domain-containing protein	-
CWD84_RS16355 (CWD84_16355)	-	3204994..3205503 (+)	510	WP_060963125.1	ferritin-like domain-containing protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141236.57 Da
• Isoelectric Point: 5.2194

>NTDB_id=257108 CWD84_RS16340 WP_060965056.1 3196879..3200583(-) (addA) [Bacillus siamensis strain SCSIO 05746]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITADEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADHFLDDLQQIEELISCRHDFEALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHVKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAADEDGRRVPSEAAVYYQEQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKVDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDELYQKLRTFYDHLQKWRSFSTNHSVAELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNREKQLAKWQAQADRADWLLSEFDRYQAASYLDFIGPALIRHRDMEAHRPPGLSAPEDIAHDPSRFHIRMLQQSE
LLEDNPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVQTLHEKELLTEEQRDAIDIEDIIQFFETEIGK
DLLGARRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAIEQITKTKVKGRALYFFDGGHVLTL

Nucleotide

• Length: 3705 bp

>NTDB_id=257108 CWD84_RS16340 WP_060965056.1 3196879..3200583(-) (addA) [Bacillus siamensis strain SCSIO 05746]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGATGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTCCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGCTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTCCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAAGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATTTGGATCTTCAAGATCTCGTCAAGCGGGTGTACGAGTA
TTCACGGTCTCATCCTGATCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAGCAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGCGCTGATCATTTTTTGGATGATCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTGAGGCGCTGTATGAACGGGTTCCCGCGGTCACATTTAAGCGGGCTAAGGCGGTAAAAG
GTGATGAATTTGACAAGGCGCTTTTGGATGAAGCGACCGATTTGCGAAACGGCGCGAAAAAACTGATCGAAAAGTTAAAG
ACCGATTACTTTACAAGAAGCCCGCAAGATCATGTAAAGAGTTTAACAGACATGAAGCCTGTCATTGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCGGCTGATGAAGACGGCCGGCGGGTGCCGAGTGAAGCGGCCGTTTATTATCAAGAGCAG
TTCCATGAGGTTCTGGTTGATGAATATCAAGATACCAATCTTGTACAGGAATCTATTCTGCAGCTTGTGAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGATGTCAAACAATCGATCTACCGATTCAGACTTGCCGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGACAGAAAGTTGACCTGAACCAAAACTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAACTCGGTGCGTCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACTGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAAT
TTTGCTGCGTTCCATGCCTTGGGCGCCGCAATTGATGGAAGAACTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CGTCGGGTTATTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCGGTCTTAAAGGTGATTGACAACCCGTATCAGGATATT
CCGCTTGCTTCCGTATTGCGCTCGCCCATTGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGACTGGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGGTGACAGACATGATGAGCTGTATCAAAAGCTGAGAACAT
TTTATGACCATCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCGGTAGCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGGTATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTGCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACTGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATATCTGCTTGATAAAGA
GCTCGGTTTCGGAACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTGCCGCTTGTGGCGATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTGTTATATGTGGCGCTGACAAGAGCGAAGGAAAAGCTGTTTCTC
ATCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCTAAATGGCAGGCGCAAGCGGACAGGGCCGACTGGCTTCTCTCTGA
ATTCGACCGCTATCAGGCGGCATCTTATTTGGACTTTATCGGGCCGGCGCTTATCCGCCACCGTGACATGGAGGCGCACA
GGCCGCCGGGTCTTTCGGCTCCGGAGGATATCGCGCACGACCCGTCCCGTTTTCATATCCGGATGCTGCAGCAAAGTGAA
CTGCTGGAAGATAACCCAAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAGGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTCCTTGAATGGGAATACCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGGGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCATATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTTCAGACGCTTCACGAAA
AAGAGCTGCTGACAGAAGAACAGCGGGACGCTATTGATATCGAAGACATTATACAGTTTTTCGAAACTGAAATCGGGAAA
GATCTTCTCGGGGCGCGGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGACTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGATTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-882)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	82.334	100	0.823