Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CWD84_RS16345 Genome accession   NZ_CP025001
Coordinates   3200570..3204070 (-) Length   1166 a.a.
NCBI ID   WP_060963123.1    Uniprot ID   A0AAI8N175
Organism   Bacillus siamensis strain SCSIO 05746     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3195570..3209070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWD84_RS16335 (CWD84_16335) sbcD 3195643..3196815 (-) 1173 WP_047476740.1 exonuclease subunit SbcD -
  CWD84_RS16340 (CWD84_16340) addA 3196879..3200583 (-) 3705 WP_060965056.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CWD84_RS16345 (CWD84_16345) addB 3200570..3204070 (-) 3501 WP_060963123.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CWD84_RS16350 (CWD84_16350) - 3204215..3204925 (+) 711 WP_060963124.1 DUF421 domain-containing protein -
  CWD84_RS16355 (CWD84_16355) - 3204994..3205503 (+) 510 WP_060963125.1 ferritin-like domain-containing protein -
  CWD84_RS16360 (CWD84_16360) - 3205712..3206335 (+) 624 WP_060963126.1 TetR/AcrR family transcriptional regulator -
  CWD84_RS16365 (CWD84_16365) - 3206351..3207547 (+) 1197 WP_060963127.1 MFS transporter -
  CWD84_RS16370 (CWD84_16370) - 3207712..3208869 (+) 1158 WP_016938296.1 AbrB family transcriptional regulator -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133424.10 Da        Isoelectric Point: 5.4513

>NTDB_id=257109 CWD84_RS16345 WP_060963123.1 3200570..3204070(-) (addB) [Bacillus siamensis strain SCSIO 05746]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKAGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQLPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SGEQAPDELNLFRMTGSTYYKLYQMAKELNADISCKELHGTKRHQHVPELACLESQYDVRPAAAYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDGGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPVQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEQMLNETREW
MAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLRAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGNGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=257109 CWD84_RS16345 WP_060963123.1 3200570..3204070(-) (addB) [Bacillus siamensis strain SCSIO 05746]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGA
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTGTTGAGGAAGCTGATTGAAGAGCATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAGCCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGTTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATCTGCATTCAGAGGATTATTT
GACGCTGCTCGCCCAGCAGCTTCCGTTAGCGGATGTTGTAAAAGGCGCCAGAGTTTACGTCGACGGCTTCTATCAATTTA
CACCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGGAGAACAGGCACCGGATGAGCTCAATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGATGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAACGGCATCAGCATGTGCCGGAGCTTGCCTGTTTAG
AATCGCAGTACGATGTGCGTCCCGCTGCGGCGTATACCGGCGGGCAGGAAGCGTTTACCGTCATACAGGCGCAAAACAGA
AGAGCCGAGCTTGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACGGAGGATACCGGTACAGGGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATAGAATTCATCCGTTCAAGTTTGGATGTCGTAAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGTGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAACTATTGTATCGCCTACGGAATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAGCAAATGCTGAATGAGACGAGAGAATGG
ATGGCCGCGCCCCTCGTCCGGCTTCAGAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCAGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCATGGGACGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACCGAATCACTTCATTTTTCTTTAATTCCCCCTGCGCTTGACCA
AGTGTTTGTAGGGAATATGGACCTGTCAAGAATGTACGGCACGTCCTGCACGTTTGTCATCGGGGCCAATGACGGCGTTC
TTCCTGCGCGCCCGGATGAAAACGGCGTGCTGTCCGATGATGACCGGGAGTGGCTGAGAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GTCATATCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATTGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAACTGTCGGCTGCTGATCCCGAGCAGGTAAGTGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCACAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTATGAAATCAGTGATGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACCGGCGCGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGTCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTCAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAGGAAATTCTGCTCAGTTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTTGTCCCGATCGGCCTTGAACTCGGTTTCGGCGGAAACGGTCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTTAGAATCGTG
GATTACAAATCAAGCGACAAGGGGCTTGATCTGGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACGTACCTTGA
CTTATCGATTACACATTCAGCGGATTGGCTCGGCATGAAAGCGACCCCCGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCAATCAGCCTGATGGACACGACTTTGGAGGAAGGACGTTCAAATATCATTAATGCCGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGATTCAGCCGCAGTCAGCGAACGGGATTTTCATCTCCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCCCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(305-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.276

100

0.833

Multiple sequence alignment