Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MFLA_RS11375 Genome accession   NC_007947
Coordinates   2376657..2377523 (+) Length   288 a.a.
NCBI ID   WP_011480446.1    Uniprot ID   Q1GZ45
Organism   Methylobacillus flagellatus KT     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2371657..2382523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS11360 (Mfla_2222) - 2373106..2374407 (-) 1302 WP_011480443.1 HlyD family secretion protein -
  MFLA_RS11365 (Mfla_2223) - 2374397..2376055 (-) 1659 WP_011480444.1 peptidase domain-containing ABC transporter -
  MFLA_RS11370 (Mfla_2224) - 2376180..2376500 (-) 321 WP_011480445.1 hypothetical protein -
  MFLA_RS11375 (Mfla_2225) pilD 2376657..2377523 (+) 867 WP_011480446.1 prepilin peptidase Machinery gene
  MFLA_RS11380 (Mfla_2226) coaE 2377527..2378135 (+) 609 WP_011480447.1 dephospho-CoA kinase -
  MFLA_RS11385 (Mfla_2227) zapD 2378190..2378948 (+) 759 WP_011480448.1 cell division protein ZapD -
  MFLA_RS14675 (Mfla_2228) - 2378936..2379124 (+) 189 WP_011480449.1 DNA gyrase inhibitor YacG -
  MFLA_RS11390 (Mfla_2229) - 2379137..2379574 (-) 438 WP_048811711.1 hypothetical protein -
  MFLA_RS11395 (Mfla_2230) - 2379565..2380497 (-) 933 WP_011480451.1 Nudix family hydrolase -
  MFLA_RS11400 (Mfla_2231) - 2380494..2381369 (-) 876 WP_011480452.1 ATP-binding protein -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31498.17 Da        Isoelectric Point: 6.3689

>NTDB_id=25664 MFLA_RS11375 WP_011480446.1 2376657..2377523(+) (pilD) [Methylobacillus flagellatus KT]
MPEFVFLDALRDNPALFITCSALLGLMVGSFLNVVIYRLPRMMELAWHAERLEAEGQPQTEQARYNLITPRSRCPHCQHA
ITALENIPIISYMMLGGKCRQCKTAISPRYPLVELLTATLTGLASWHFGYSSLAVASWLIIFALIALTFIDLDTHLLPDD
ITLPLLWAGLLFNLDHGFTDISSAVIGAAAGYLVLWSVYWLFKLITGKEGMGYGDFKLLAALGAWFGWQLLPAVILLSSV
AGSIIGIGLIVLTKHGRETPIPFGPYLALGGIAALFCGEQLSQLYLGG

Nucleotide


Download         Length: 867 bp        

>NTDB_id=25664 MFLA_RS11375 WP_011480446.1 2376657..2377523(+) (pilD) [Methylobacillus flagellatus KT]
ATGCCTGAATTTGTTTTTCTGGATGCTTTGAGAGACAACCCCGCCCTCTTCATTACCTGCTCTGCGCTCCTGGGCCTCAT
GGTCGGCAGCTTCCTCAACGTGGTGATATACCGGTTGCCACGCATGATGGAGCTGGCCTGGCATGCTGAGCGCTTGGAAG
CCGAAGGCCAACCTCAAACAGAACAGGCCCGCTACAACCTGATCACACCTCGGTCACGCTGCCCGCATTGCCAGCATGCC
ATCACAGCACTGGAAAATATACCAATCATCAGCTACATGATGCTGGGAGGAAAATGCAGGCAATGCAAGACTGCAATCAG
CCCGCGATACCCTTTGGTAGAACTCCTCACGGCAACGCTGACCGGTCTTGCCAGCTGGCATTTCGGCTATTCCAGCCTTG
CCGTGGCAAGCTGGCTTATCATCTTCGCGCTGATCGCACTCACCTTTATCGACCTCGACACGCATCTGCTGCCAGACGAT
ATCACCTTGCCCTTGCTCTGGGCAGGACTGCTGTTCAATCTCGACCATGGCTTCACCGACATCAGCTCTGCCGTGATTGG
GGCTGCTGCCGGATATCTTGTGTTGTGGTCAGTATACTGGCTATTCAAGCTCATCACAGGCAAAGAGGGCATGGGCTACG
GTGACTTCAAGCTCTTGGCAGCCCTGGGAGCCTGGTTCGGCTGGCAATTGCTCCCTGCTGTCATTTTATTATCATCCGTC
GCTGGTTCCATCATCGGAATTGGATTGATCGTATTGACCAAGCACGGACGGGAAACCCCGATTCCATTCGGGCCGTACCT
GGCACTGGGAGGAATCGCAGCCCTGTTCTGCGGTGAACAACTTTCTCAATTATATTTAGGAGGCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(23-128)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1GZ45

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

52.817

98.611

0.521
  pilD Vibrio cholerae strain A1552

53.791

96.181

0.517
  pilD Acinetobacter baumannii D1279779

49.11

97.569

0.479
  pilD Acinetobacter nosocomialis M2

48.754

97.569

0.476
  pilD Neisseria gonorrhoeae MS11

51.383

87.847

0.451

Multiple sequence alignment