Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   CTW00_RS00355 Genome accession   NZ_CP024843
Coordinates   62868..64166 (-) Length   432 a.a.
NCBI ID   WP_003101738.1    Uniprot ID   -
Organism   Streptococcus iniae strain FP5228     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 60346..66480 62868..64166 within 0


Gene organization within MGE regions


Location: 60346..66480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTW00_RS00340 (CTW00_00069) - 60346..61475 (-) 1130 Protein_61 IS3 family transposase -
  CTW00_RS00345 (CTW00_00071) raiA 61584..62132 (-) 549 WP_003101732.1 ribosome-associated translation inhibitor RaiA -
  CTW00_RS00350 (CTW00_00072) - 62207..62692 (-) 486 WP_003101735.1 ComF family protein -
  CTW00_RS00355 (CTW00_00073) comFA/cflA 62868..64166 (-) 1299 WP_003101738.1 DEAD/DEAH box helicase Machinery gene
  CTW00_RS00360 (CTW00_00074) - 64222..64860 (+) 639 WP_003101740.1 YigZ family protein -
  CTW00_RS00365 (CTW00_00075) cysK 64957..65883 (+) 927 WP_003101742.1 cysteine synthase A -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49813.92 Da        Isoelectric Point: 9.3172

>NTDB_id=255789 CTW00_RS00355 WP_003101738.1 62868..64166(-) (comFA/cflA) [Streptococcus iniae strain FP5228]
MESLELYYGRIFTSKQLSEVEKVKAEKIDSMFKIGRHWYCQRCRMKIDLNNKLPSGKYYCRECLIFGRNESDDFLYFFDS
LPFEAGTYLKWQGQLTQYQEAVSQQLLKHFKNNQRSLVHAVTGAGKTEMIYQLIAYVLEKGGWLCLACPRVDVCIELEKR
ISRDFSCSVTLMHAHSESYQRNPIIIATTHQLLKFYRAFDLIIIDEVDAFPFVNNKMLNQALENALAPSGKIVYLTATST
KKLDKDVKNGRCQGITLARRFHNNPLVLPEFQLVLSLSEKLKRHKLPRNIKKQLKQQRLSKHPLLIFFPIIEDGQLFQDL
LTTHFPDEQIAFVSSESEERAELIESFRNKEISILISTTILERGVTFPGVDVFVILANHRLYNASSLIQIAGRVGRSIER
PEGKLLFFHEGISVAMIKAKSEIIKMNKIAYG

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=255789 CTW00_RS00355 WP_003101738.1 62868..64166(-) (comFA/cflA) [Streptococcus iniae strain FP5228]
ATGGAAAGTTTAGAATTATATTATGGAAGAATTTTTACCAGTAAACAATTATCTGAAGTAGAAAAAGTCAAGGCTGAAAA
AATAGATAGTATGTTTAAGATAGGACGCCACTGGTATTGCCAGCGGTGCAGAATGAAGATTGATTTAAATAATAAACTTC
CTTCTGGAAAATATTACTGTAGAGAGTGTCTTATTTTTGGCCGTAACGAAAGTGATGATTTTCTTTATTTTTTTGACAGT
TTACCATTTGAAGCTGGAACTTACCTTAAGTGGCAAGGGCAACTGACACAATACCAAGAAGCAGTCTCTCAACAGCTCCT
TAAACATTTTAAAAATAATCAGCGAAGTTTAGTACACGCTGTCACTGGGGCAGGAAAAACCGAAATGATTTATCAATTAA
TAGCATATGTTCTTGAAAAAGGAGGATGGCTTTGTTTGGCATGTCCAAGAGTTGATGTATGTATAGAACTTGAAAAGAGA
ATTTCACGCGATTTTTCTTGTTCAGTAACCTTAATGCATGCACATTCAGAAAGTTATCAGAGAAATCCAATCATTATTGC
CACAACACATCAATTGTTAAAGTTTTACCGGGCTTTTGATTTAATTATTATTGATGAAGTTGATGCCTTTCCATTCGTTA
ATAATAAAATGCTTAACCAAGCCCTTGAAAATGCTTTAGCTCCAAGTGGGAAAATAGTTTACTTAACAGCAACCTCAACT
AAAAAACTAGATAAAGATGTTAAAAATGGAAGATGTCAAGGGATTACATTAGCAAGGAGATTCCACAATAATCCATTGGT
TCTACCAGAATTTCAATTAGTTTTATCGTTATCTGAGAAATTGAAAAGACATAAGTTACCAAGGAATATAAAAAAACAGC
TTAAACAACAACGGCTAAGTAAACATCCCTTACTCATTTTCTTCCCTATTATTGAGGATGGCCAATTATTTCAAGACCTA
TTAACAACCCATTTTCCAGATGAACAGATTGCATTTGTTTCCAGTGAATCTGAAGAAAGAGCTGAGCTTATTGAATCTTT
TAGAAATAAGGAAATATCCATTTTAATTTCAACAACCATATTAGAAAGGGGTGTTACATTTCCAGGTGTAGATGTTTTTG
TTATTCTAGCCAACCATAGGTTGTATAATGCTTCTTCGCTTATTCAGATAGCAGGGCGAGTTGGCCGTTCGATAGAAAGA
CCTGAAGGAAAATTACTCTTTTTTCATGAAGGGATAAGCGTAGCTATGATAAAAGCCAAAAGTGAAATAATAAAGATGAA
TAAGATTGCTTATGGCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

52.123

98.148

0.512
  comFA/cflA Streptococcus pneumoniae Rx1

52.123

98.148

0.512
  comFA/cflA Streptococcus pneumoniae D39

52.123

98.148

0.512
  comFA/cflA Streptococcus pneumoniae R6

52.123

98.148

0.512
  comFA/cflA Streptococcus mitis NCTC 12261

51.651

98.148

0.507
  comFA/cflA Streptococcus mitis SK321

50.708

98.148

0.498
  comFA Lactococcus lactis subsp. cremoris KW2

49.49

90.741

0.449
  comFA Latilactobacillus sakei subsp. sakei 23K

35.945

100

0.361

Multiple sequence alignment