Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CUB85_RS12705 Genome accession   NZ_CP024797
Coordinates   2577656..2578726 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain TJ02     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2572656..2583726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUB85_RS12655 sipW 2572716..2573300 (-) 585 WP_007408328.1 signal peptidase I SipW -
  CUB85_RS12660 tapA 2573272..2573943 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  CUB85_RS12665 - 2574202..2574531 (+) 330 WP_020954231.1 DUF3889 domain-containing protein -
  CUB85_RS12670 - 2574571..2574750 (-) 180 WP_003153093.1 YqzE family protein -
  CUB85_RS12675 comGG 2574807..2575184 (-) 378 WP_039063315.1 competence type IV pilus minor pilin ComGG -
  CUB85_RS12680 comGF 2575185..2575685 (-) 501 WP_258038902.1 competence type IV pilus minor pilin ComGF -
  CUB85_RS12685 comGE 2575594..2575908 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  CUB85_RS12690 comGD 2575892..2576329 (-) 438 WP_007408322.1 competence type IV pilus minor pilin ComGD Machinery gene
  CUB85_RS12695 comGC 2576319..2576627 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  CUB85_RS12700 comGB 2576632..2577669 (-) 1038 WP_032866436.1 competence type IV pilus assembly protein ComGB Machinery gene
  CUB85_RS12705 comGA 2577656..2578726 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  CUB85_RS12710 - 2578918..2579868 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  CUB85_RS12715 - 2580014..2581315 (+) 1302 WP_039063318.1 hemolysin family protein -
  CUB85_RS12720 - 2581353..2582183 (-) 831 WP_015240214.1 STAS domain-containing protein -
  CUB85_RS12730 - 2582390..2582770 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  CUB85_RS12735 - 2583007..2583252 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=255507 CUB85_RS12705 WP_012117985.1 2577656..2578726(-) (comGA) [Bacillus velezensis strain TJ02]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=255507 CUB85_RS12705 WP_012117985.1 2577656..2578726(-) (comGA) [Bacillus velezensis strain TJ02]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCCTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment